גילוי אינטראקציות חלבון ואפיון תפקוד חלבון באמצעות HaloTag טכנולוגיה

1:23

Protein Pulldowns: Preparation of Transiently Transfected Cells

2:50

Equilibration of the HaloLink Resin

4:01

Binding and Washing of Fusion Complexes

6:21

SDS Elution for Denaturing Gels, Western Blots, or Mass Spectrometry

7:07

Cellular Imaging of Fluorescently Labeled Fusion Proteins Using a Cytoplasmic and Nuclear Permeable Ligand

8:48

Results: Using HaloTag Technology to Determine Protein Interactions and Localization

10:47

Conclusion