Shanghai Jiaotong University School of Medicine 6 articles published in JoVE Developmental Biology A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation Qinggang Dai*1, Yujiao Han*2, Furong Xie*1, Xuhui Ma3, Zhan Xu2, Xiao Liu1, Weiguo Zou2, Jun Wang1 1Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine This manuscript describes a protocol to isolate and culture osteoclasts in vitro from mouse bone marrow, and to study the role of the mammalian/mechanistic target of rapamycin complex 1 in osteoclast formation. Medicine Surgical Treatment for Benign Prostatic Hyperplasia: Holmium Laser Enucleation of the Prostate (HoLEP). Huan Xu*1, Xiang Wan*1, Meng Gu1, Yanbo Chen1, Qiling Shi1, Qi Chen1, Zhong Wang1 1Department of Urology, Shanghai 9th People's Hospital, Shanghai Jiaotong University School of Medicine Here we present a safe and efficient protocol, holmium laser enucleation of the prostate, to treat benign prostatic hyperplasia. Medicine A Detailed Protocol for Physiological Parameters Acquisition and Analysis in Neurosurgical Critical Patients Xiang Wu1,2, Guoyi Gao1,2, Junfeng Feng1,2, Qing Mao1,2, Jiyao Jiang1,2 1Department of Neurosurgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, 2Shanghai Institute of Head Trauma Recently, we have developed a portable multimodality monitoring system for the monitoring of various physiological parameters in neurosurgical critical patients. Detailed protocols on how to use this multimodality monitoring system are presented here. Neuroscience In Vitro Characterization of the Electrophysiological Properties of Colonic Afferent Fibers in Rats Youqiang Meng1, Li Dong1, Biying Sun1, Ping Luo1, Guohua Zhang1, Weifang Rong1 1Hongqiao International Institute of Medical Research, Tongren Hospital and Department of Physiology, Faculty of Basic Medical Sciences, Shanghai Jiaotong University School of Medicine Abnormal sensory function underlies visceral pain and other symptoms of functional and inflammatory bowel diseases. A protocol for the electrophysiological recording of the colonic afferent nerves in an ex vivo rat colorectum preparation is presented here. Developmental Biology Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta Ashish Misra1, Zhonghui Feng1, Jiasheng Zhang1, Zhi-Yin Lou2,3, Daniel M. Greif1 1Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, 2Department of Neurology, Yale University School of Medicine, 3Department of Neurology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine Protocols for studying the embryonic and perinatal murine aorta using in vivo clonal analysis and fate mapping, aortic explants, and isolated smooth muscle cells are detailed here. These diverse approaches facilitate the investigation of the morphogenesis of the embryonic and perinatal aorta in normal development and the pathogenesis in disease. Developmental Biology Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System Shenglan Li*1,2, Haipeng Xue*1,2, Bo Long1,2,5, Li Sun1,2,6, Tai Truong1,2,4,7, Ying Liu1,2,3 1Department of Neurosurgery, The University of Texas Health Science Center at Houston, 2Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, 3The Senator Lloyd & B. A. Bentsen Center for Stroke Research, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, 4Summer Research Program, Office of Educational Programs, The University of Texas Health Science Center at Houston, 5Department of Anesthesiology, Shengjing Hospital, China Medical University, 6Department of Oncology, Renji Hospital, Shanghai Jiaotong University School of Medicine, 7Biology Department, University of West Georgia Genome editing tools such as the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system have greatly improved gene targeting efficiency in human induced pluripotent stem cells (hiPSCs). This manuscript describes a protocol for generating lineage specific hiPSC reporter using CRISPR/Cas system assisted homologous recombination.