Guangzhou Medical University 14 articles published in JoVE Neuroscience Dual-Task Stroop Paradigm for Detecting Cognitive Deficits in High-Functioning Stroke Patients Songjun Lin1,2, Qiang Lin3,4,5, Biyi Zhao3,4,6, Yongchun Jiang3,4, Wanqi Zhuang3,4, Delong Chen3,4, Yajie Zhang3,4, Aijia Chen3,4, Qianrong Zhang7, Yuxin Zheng3,4, Jianjun Wang1, Fangqiu Xu8, Xi Qin3,4,9, Yefeng Cai10 1Department of Neurology and Psychology, the Fourth Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen Traditional Chinese Medicine Hospital, 2Guangzhou University of Chinese Medicine, 3Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, 4Department of Rehabilitation Medicine, Guangzhou Medical University, 5Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, 6School of Traditional Chinese Medicine, Jinan University, 7Health College of Guangdong Pharmaceutical University, 8Department of Clinical Medicine, Guangzhou Medical University, 9Department of Rehabilitation Medicine, Panzhihua Central Hospital, 10Department of Neurology and Psychology, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Clinical assessment scales are notsensitive enough to cognitive dysfunction in high-functioning stroke patients. The dual-task paradigm presents advantages and potential in the assessment and cognitive training of cognitive dysfunction. The study here proposes a dual-task Stroop paradigm to identify cognitive dysfunction in high-functioning stroke patients. Medicine A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis Yan Tian*1, Junyu Huang*1, Ming Fu1, Qiuming He1, Jiale Chen1, Yan Chen1, Ruizhong Zhang1, Wei Zhong1 1Provincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University Necrotizing enterocolitis (NEC) is the most severe gastrointestinal (GI) disease that often occurs in premature infants, especially very low birth weight infants, with high mortality and unclear pathogenesis. The cause of NEC may be related to inflammatory immune regulatory system abnormalities. An NEC animal model is an indispensable tool for NEC disease immune research. NEC animal models usually use C57BL/6J neonatal mice; BALB/c neonatal mice are rarely used. Related studies have shown that when mice are infected, Th2 cell differentiation is predominant in BALB/c mice compared to C57BL/6J mice. Studies have suggested that the occurrence and development of NEC are associated with an increase in T helper type 2 (Th2) cells and are generally accompanied by infection. Therefore, this study used neonatal BALB/c mice to induce an NEC model with similar clinical characteristics and intestinal pathological changes as those observed in children with NEC. Further study is warranted to determine whether this animal model could be used to study Th2 cell responses in NEC. Neuroscience Motor Dual-Tasks for Gait Analysis and Evaluation in Post-Stroke Patients Haining Ou*1,2,3,4, Shijuan Lang*1,2,3, Yuxin Zheng1,2,3, Dongqing Huang2, Sheng Gao1, Meifeng Zheng1,2, Biyi Zhao2, Zulipiya Yiming2, Yaxian Qiu1,2, Qiang Lin*1,2,3,4, Junjie Liang*1,2,3 1Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, 2Department of Rehabilitation Medicine, Guangzhou Medical University, 3Rehabilitation Medicine Lab, Guangzhou Medical University, 4Guangzhou Key Laboratory of Enhanced Recovery after Abdominal Surgery, The Fifth Affiliated Hospital of Guangzhou Medical University This paper presents a protocol specifically for dual motor task gait analysis in stroke patients with motor control deficits. Biology Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae Chuanxian Wei*1, Yan Yan*1, Xiaoli Miao1, Renjie Jiao1,2,3 1Sino-French Hoffmann Institute, School of Basic Medical Sciences, Guangzhou Medical University, 2The Second Affiliated Hospital of Guangzhou Medical University, 3The State Key Laboratory of Respiratory Disease, Guangzhou Medical University Presented here are detailed methods for the dissection and lipid droplet staining of oenocytes in Drosophila larvae using BODIPY 493/503, a lipid droplet-specific fluorescent dye. Medicine The Lower Body Positive Pressure Treadmill for Knee Osteoarthritis Rehabilitation Junjie Liang*1,2, Yuanyuan Guo*1,2, Yuxin Zheng1,2, Shijuan Lang1, Hongxin Chen1,2, Yaoyao You1,2, Bryan O’Young3,4, Haining Ou*1,2, Qiang Lin*1,2 1Department of Rehabilitation, The Fifth Affiliated Hospital of Guangzhou Medical University, 2Experiment Education Model Center of Rehabilitation Medicine, Guangzhou Medical University, 3Department of Physical Medicine and Rehabilitation, Geisinger Health System, 4Department of Rehabilitation Medicine, New York University School of Medicine Here, based on a clinician’s point-of-view, we propose a two-model lower body positive pressure (LBPP) protocol (walking and squatting models) in addition to a clinical, functional assessment methodology, including details for further encouragement of the development of non-drug surgical intervention strategies in knee osteoarthritis patients. However, we only present the effect of LBPP training in improvement of pain and knee function in one patient through three-dimensional gait analysis. The exact, long-term effects of this approach should be explored in future studies. Biology Induction of Right Ventricular Failure by Pulmonary Artery Constriction and Evaluation of Right Ventricular Function in Mice Qiancheng Wang1, Kaitong Chen1, Hairuo Lin1, Mingyuan He2, Xiaoxia Huang1, Hailin Zhu1, Yulin Liao1 1Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, 2The Third Clinical Medical College, Guangzhou Medical University Here, we provide a useful approach for studying the mechanism of right ventricular failure. A more convenient and efficient approach to pulmonary artery constriction is established using surgical instruments made inhouse. In addition, methods to evaluate the quality of this approach by echocardiography and catheterization are provided. Neuroscience Diffusion Tensor Magnetic Resonance Imaging in Chronic Spinal Cord Compression Weipeng Zheng*1, Xiuhang Ruan*2, Xinhua Wei3, Fangtian Xu4, Yuanping Huang1, Ning Wang1, Haoyi Chen5, YingJie Liang6, Wende Xiao6, Xin Jiang1, Shifeng Wen6 1Department of Orthopedics, Guangzhou First People's Hospital, Guangzhou Medical University, 2Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, 3Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, 4Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, 5Department of Orthopedics, Guangzhou Chest Hospital, 6Department of Orthopedics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou Medical University Here, we present a protocol for the application of diffusion tensor imaging parameters to evaluate spinal cord compression. Behavior Simultaneous Recordings of Cortical Local Field Potentials and Electrocorticograms in Response to Nociceptive Laser Stimuli from Freely Moving Rats Lupeng Yue1,2, Fengrui Zhang3, Xuejing Lu1,2, You Wan4,5,6, Li Hu1,2,7 1CAS Key Laboratory of Mental Health, Institute of Psychology, 2Department of Psychology, University of Chinese Academy of Sciences, 3Research Center of Brain Cognitive Neuroscience, Liaoning Normal University, 4Neuroscience Research Institute, Peking University, 5Department of Neurobiology, School of Basic Medical Sciences, Peking University, 6Key Laboratory for Neuroscience, Ministry of Education/National Health and Family Planning Commission, Peking University, 7Department of Pain Management, State Key Clinical Specialty in Pain Medicine, Second Affiliated Hospital of Guangzhou Medical University We developed a technique that simultaneously records both electrocorticography and local field potentials in response to nociceptive laser stimuli from freely moving rats. This technique helps establish a direct relationship of electrocortical signals at the mesoscopic and macroscopic levels, which facilitates the investigation of nociceptive information processing in the brain. Immunology and Infection A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice Ming Fu1, Zefeng Lin2, Huiting Lin2, Yanlu Tong2, Hezhen Wang2, Hongjiao Chen2, Yan Chen2, Ruizhong Zhang2 1First Affiliated Hospital of Jinan University, 2Department of Pediatric Surgery, Guangzhou Women & Children's Medical Center, Guangzhou Medical University This article describes in detail a method based on silver nanoparticles for ameliorating biliary atresia syndrome in an experimental biliary atresia mouse model. A solid understanding of the reagent preparation process and the neonatal mouse injection technique will help familiarize researchers with the method used in neonatal mouse model studies. Developmental Biology A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes Jiayin Yang*1,2,6, Lai-Yung Wong*2, Xiao-Yu Tian*3, Rui Wei1,2, Wing-Hon Lai2, Ka-Wing Au2, Zhiwei Luo4,5, Carl Ward4,5, Wai-In Ho2, David P. Ibañez4,5, Hao Liu4,5, Xichen Bao4,5, Baoming Qin4,5, Yu Huang3, Miguel A. Esteban4,5,7, Hung-Fat Tse1,2,6,7 1Department of Medicine, University of Hong Kong-Shenzhen Hospital, 2The Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, 3School of Biomedical Sciences, Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, 4Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University, 5Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 6Research Centre of Heart, Brain, Hormone, and Healthy Ageing, Li Ka Shing Faculty of Medicine, University of Hong Kong, 7Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre, University of Hong Kong and Guangzhou Institutes of Biomedicine and Health Here, we present a protocol to generate a human liver chimeric mouse model of familial hypercholesterolemia using human induced pluripotent stem cell-derived hepatocytes. This is a valuable model for testing new therapies for hypercholesterolemia. Biology Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model Zhaofeng Jia1,2,3, Yujie Liang4,5, Xingfu Li2,3, Xiao Xu1,2,3, Jianyi Xiong2,3, Daping Wang1,2,3, Li Duan2,3 1Postgraduate institution, Guangzhou Medical University, 2Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, 3 This article presents a simple and economic protocol for the straightforward isolation and purification of mesenchymal stem cells from New Zealand white rabbit synovial fluid. Developmental Biology Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification Cui Lin1, Dongxing Zhu2, Greg Markby1, Brendan M. Corcoran3, Colin Farquharson1, Vicky E. Macrae1 1Developmental Biology, The Roslin Institute and R(D)SVS, University of Edinburgh, 2Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences, Guangzhou Medical University, 3Clinical Sciences and R(D)SVS, University of Edinburgh This protocol describes the isolation, culture, and calcification of rat-derived valve interstitial cells, a highly physiological in vitro model of calcific aortic valve disease (CAVD). Exploitation of this rat model facilitates CAVD research in exploring the cell and molecular mechanisms that underlie this complex pathological process. Medicine A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma Wenting Luo*1,2,3,4, Guichang Pan*5, Huimin Huang1,2,3,4, Peiyan Zheng1,2,3,4, Nili Wei1,2,3,4, Yifei Zhang5, Guangqiao Zeng1,2,3,4, Baoqing Sun1,2,3,4 1State Key Laboratory of Respiratory Disease, 2National Clinical Research Center of Respiratory Disease, 3Guangzhou Institute of Respiratory Diseases, 4Department of Allergy and Clinical Immunology, First Affiliated Hospital of Guangzhou Medical University, 5Department of Respiratory Medicine, Sixth Affiliated Hospital of Guangzhou Medical University This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma. Developmental Biology A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro Zhaofeng Jia1,2, Yujie Liang3, Bin Ma4,5, Xiao Xu2,6, Jianyi Xiong2, Li Duan2, Daping Wang1,2 1Guangzhou Medical University, 2 We present a method to quantify DNA methylation based on the 5-methylcytosine (5-mC) dot blot. We determined the 5-mC levels during chondrocyte dedifferentiation. This simple technique could be used to quickly determine the chondrocyte phenotype in ACI treatment.