Ministry of Education 6 articles published in JoVE Medicine Clinical Efficacy of Small Needle Knife Therapy on Stage I-II Frozen Shoulder Suxia Guo1, Dan Liu1, Yanping Yang1,2, Zhijun Hu1 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of theory and therapy of muscles and bones, Ministry of Education The protocol presented here describes the detailed operation method and proves the effectiveness of a small needle knife in the treatment of frozen shoulders. Cancer Research RNA Fluorescence In Situ Hybridization for Long Non-Coding RNA Localization in Human Osteosarcoma Cells Junli Chang*1,2, Xiaoping Ma*1,2, Xingyuan Sun1,2, Chujie Zhou1,2, Peng Zhao1,2, Yongjun Wang1,2, Yanping Yang1,2 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education The present protocol describes a method of RNA fluorescence in situ hybridization to localize the lncRNAs in human osteosarcoma cells. Biology Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e Shaohui Zheng1,2, Fang Liang3, Yu Zhang1,2, Ji-Feng Fei4, Wei Qin1,2, Yanmei Liu1,2 1Key Laboratory of Brain, Cognition and Education Science, Ministry of Education, 2Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, 3Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Engineering Technology Research Center for Environmentally Friendly Aquaculture, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Sciences, South China Normal University, 4Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences This protocol describes how to perform efficient adenine base editing without PAM limitation to construct a precise zebrafish disease model using zSpRY-ABE8e. Cancer Research Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model Junli Chang*1,2, Xingyuan Sun*1,2, Xiaoping Ma1,2, Peng Zhao1,2, Binhao Shi1,2, Yongjun Wang1,2, Xianghui Han1,3, Yanping Yang1,2 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education, 3Institute of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine Here, we present a protocol for the intra-cardiac injection of human prostate cancer cells to generate a mouse model with bone metastasis lesions. Medicine The Miniature Pig: A Large Animal Model for Cochlear Implant Research Xiaojun Ji*1,2,3,4, Yi Luo*1,2,3,4, Weiwei Guo1,2,3,4, Fei Ji1,2,3,4, Shuolong Yuan1,2,3,4, Liangwei Xu1,2,3,4, Wei Chen*1,2,3,4 1College of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, 2National Clinical Research Center for Otolaryngologic Diseases, 3Key Lab of Hearing Science, Ministry of Education, 4Beijing Key Lab of Hearing Impairment for Prevention and Treatment Miniature pigs (mini-pigs) are an ideal large animal model for research into cochlear implants. Cochlear implantation surgery in mini-pigs can be utilized to provide initial evidence of the safety and potential performance of novel electrode arrays and surgical approaches in a living system similar to human beings. Developmental Biology Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies Wei Zheng1,2, Zhiguo Chen1,2 1Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, 2Center of Neural Injury and Repair, Beijing Institute for Brain Disorders The protocol presents the reprogramming of peripheral blood mononuclear cells to induce neural stem cells by Sendai virus infection, differentiation of iNSCs into dopaminergic neurons, transplantation of DA precursors into the unilaterally-lesioned Parkinson's disease mouse models, and evaluation of the safety and efficacy of iNSC-derived DA precursors for PD treatment.
