People's Hospital of Longhua 1 article published in JoVE Biology Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs Yanli Zhao1,2,3, Chengjiang Zhao4, David K.C. Cooper5, Ying Lu2, Kewang Luo6, Huiyun Wang3, Pengfei Chen3, Changchun Zeng3, Shaodong Luan1, Lisha Mou2, Hanchao Gao1,2,3 1Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 2Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, 3Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 4Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 5Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, 6 An effective enzymatic method for isolation of primary porcine aortic endothelial cells (pAECs) from miniature pigs is described. The isolated primary pAECs can be used to investigate the immune and coagulation response in xenotransplantation.
