The University of Hong Kong 3 articles published in JoVE Medicine Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies Yichi Zhang1,2,3, Kaiqi Long1,2,3, Weiping Wang1,2,3 1State Key Laboratory of Pharmaceutical Biotechnology, The University of Hong Kong, 2Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 3Laboratory of Molecular Engineering and Nanomedicine, Dr. Li Dak-Sum Research Centre, The University of Hong Kong This protocol describes the fabrication and characterization of a photoresponsive prodrug-dye nanoassembly. The methodology for drug release from the nanoparticles by light-triggered disassembly, including the light irradiation setup, is explicitly described. The drugs released from the nanoparticles following light irradiation exhibited excellent anti-proliferation effects on human colorectal tumor cells. Cancer Research Non-Invasive PET/MR Imaging in an Orthotopic Mouse Model of Hepatocellular Carcinoma Kel Vin Tan*1,2, Xinxiang Yang*3, Chung Ying Chan2, Jingjing Shi1, Hing-Chiu Chang4, Keith Wan-Hang Chiu1,5, Kwan Man3 1Department of Diagnostic Radiology, School of Clinical Medicine, LKS Faculty of Medicine, The University of Hong Kong, 2Department of Oncology, MRC Oxford Institute for Radiation Oncology, University of Oxford, 3Department of Surgery, School of Clinical Medicine, HKU-SZH & LKS Faculty of Medicine, The University of Hong Kong, 4Department of Biomedical Engineering, The Chinese University of Hong Kong, 5Department of Diagnostic and Interventional Radiology, Kwong Wah Hospital Here, we present a protocol to create orthotopic hepatocellular carcinoma xenografts with and without hepatic artery ligation and perform non-invasive positron emission tomography (PET) imaging of tumor hypoxia using [18F]Fluoromisonidazole ([18F]FMISO) and [18F]Fluorodeoxyglucose ([18F]FDG). Biology Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids Cun Li1,2, Man Chun Chiu1,2, Yifei Yu1,2, Xiaojuan Liu1,2, Ding Xiao1,2, Jingjing Huang1,2, Zhixin Wan1,2, Jie Zhou1,2,3 1Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 2Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, 3State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong The protocol presents a method to derive human lung organoids from primary lung tissues, expand the lung organoids and induce proximal differentiation to generate 3D and 2D airway organoids that faithfully phenocopy the human airway epithelium.
