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Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies
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JoVE Journal Medicine
Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies

Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies

DOI:
10.3791/64677-v

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08:54 min

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February 17, 2023

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, ,
1State Key Laboratory of Pharmaceutical Biotechnology,The University of Hong Kong, 2Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine,The University of Hong Kong, 3Laboratory of Molecular Engineering and Nanomedicine, Dr. Li Dak-Sum Research Centre,The University of Hong Kong

Chapters

  • 00:04Introduction
  • 00:37Preparation of IR783/BC Nanoparticles by the Flash Precipitation Method
  • 02:52Characterization of IR783/BC Nanoparticles
  • 04:47Photoactivation of IR783/BC Nanoparticles
  • 06:24Results: Fabrication and Characterization of a Photoresponsive Prodrug-Dye Nano Assembly
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the fabrication and characterization of a photoresponsive prodrug-dye nanoassembly. The methodology for drug release from the nanoparticles by light-triggered disassembly, including the light irradiation setup, is explicitly described. The drugs released from the nanoparticles following light irradiation exhibited excellent anti-proliferation effects on human colorectal tumor cells.

Tags

Prodrug-dye NanoassembliesPhotoactivationDrug DeliveryColorectal TumorBoron Dipyrromethene ChlorambucilIR783Nanoparticle FabricationEncapsulation EfficiencyLoading CapacityDynamic Light ScatteringSurface Charge

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