Becton Dickinson Medical Devices (Shanghai) Co Ltd 2 articles published in JoVE Immunology and Infection Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children Huizheng Ge*1, Linlin Zhang*2,3, Mengjia Liu*2,3, Xi Yang1, Xu Wu1, Ran Wang2,3, Zhengde Xie2,3 1Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd, 2 In this study, a method was developed to facilitate the transfer of experimental settings and analysis templates between two flow cytometers in two laboratories for the detection of lymphocytes in Japanese encephalitis-vaccinated children. The standardization method for the flow cytometer experiments will allow research projects to be effectively conducted in multiple centers. Immunology and Infection Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique Linlin Zhang*1,2, Meng Zhang*1,2,3, Mengjia Liu1,2, Junhong Ai1,2, Jiao Tian1,2, Huizheng Ge4, Ran Wang1,2, Zhengde Xie1,2 1 The present protocol combines ex vivo stimulation and flow cytometry to analyze polyfunctional T cell (TPF) profiles in peripheral blood mononuclear cells (PBMCs) within Japanese encephalitis virus (JEV)-vaccinated children. The detection method and flow cytometry color scheme of JEV-specific TPFs were tested to provide a reference for similar studies.
