Kaohsiung Medical University 7 articles published in JoVE Medicine Investigation of the Electrophysiological and Thermographic Safety Parameters of Surgical Energy Devices During Thyroid and Parathyroid Surgery in a Porcine Model Hsin-Yi Tseng1, Tzu-Yen Huang1, Jia Joanna Wang1,2, Yi-Chu Lin1, I-Cheng Lu3, Feng-Yu Chiang4, Gianlorenzo Dionigi5,6, Gregory W. Randolph7, Che-Wei Wu1,8 1Department of Otorhinolaryngology-Head and Neck Surgery, International Thyroid Surgery Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 2Department of Otolaryngology-Head and Neck Surgery, Kaohsiung Municipal Siaogang Hospital, Kaohsiung Medical University, 3Department of Anesthesiology, Kaohsiung Municipal Siaogang Hospital, Kaohsiung Medical University, 4Department of Otolaryngology-Head and Neck Surgery, E-Da Hospital, I-Shou University, 5Division of General Surgery, Endocrine Surgery Section, Istituto Auxologico Italiano (IRCCS), 6Department of Pathophysiology and Transplantation, Faculty of Medicine and Surgery, University of Milan, 7Division of Thyroid and Parathyroid Endocrine Surgery, Department of Otolaryngology-Head and Neck Surgery, Massachusetts Eye and Ear Infirmary, Harvard Medical School, 8Center for Liquid Biopsy and Cohort Research, and Faculty of Medicine, College of Medicine, Kaohsiung Medical University The safe application of newly developed surgical energy devices in thyroid/parathyroid surgery attracts the attention of surgeons. Animal experimental models can avoid unnecessary trials and errors in human surgery. This report aims to demonstrate electrophysiological and thermographic methods to evaluate the safety parameters of SEDs in thyroid/parathyroid surgery. Immunology and Infection Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16 Jonathan Y. Wang1, Chien-Ju Lin2, Ching-Hsuan Liu3,4, Liang-Tzung Lin3,5 1Department of Molecular Biosciences, University of Texas at Austin, 2School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, 3Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, 4Department of Microbiology and Immunology, Dalhousie University, 5Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University The goal of the protocol is to illustrate the different assays relating to viral entry that can be used to identify candidate viral entry inhibitors. Biology Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions Yung-Chia Chen1,2, Bu-Miin Huang3 1Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, 2Department of Anatomy, School of Medicine, Kaohsiung Medical University, 3Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University The hormone secreted by the adrenal cortex is vital for animals against stress and diseases. Here, we present a protocol to culture the primary rat adrenal cells. It could be a good in vitro platform for investigating the mechanisms of the reagent of interest in adrenal steroidogenesis and lipid biosynthesis. Medicine Intra-Operative Neural Monitoring of Thyroid Surgery in a Porcine Model Che-Wei Wu1,2,3, Tzu-Yen Huang2, Hui-Chun Chen4, Hsiu-Ya Chen5, Tsung-Yi Tsai2, Pi-Ying Chang5, Yi-Chu Lin2, Hsin-Yi Tseng2, Pao-Chu Hun6, Xiaoli Liu7, Hui Sun7, Gregory W. Randolph8, Gianlorenzo Dionigi9, Feng-Yu Chiang2,3, I-Cheng Lu5,10,11 1Department of Otorhinolaryngology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University, 2Department of Otorhinolaryngology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 3Department of Otorhinolaryngology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, 4Department of Nursing, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 5Department of Anesthesiology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 6Laboratory Animal Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 7Department of Thyroid and Parathyroid Surgery, China-Japan Union Hospital and Jilin Provincial Key Laboratory of Surgical Translational Medicine, Jilin University, 8Division of Thyroid and Parathyroid Endocrine Surgery, Department of Otolaryngology, Massachusetts Eye and Ear Infirmary; Division of Surgical Oncology, Department of Surgery, Massachusetts General Hospital; Department of Otology and Laryngology, Harvard Medical School, 9Division for Endocrine Surgery, Department of Human Pathology in Adulthood and Child-hood "G. Barresi", University Hospital G. Martino, University of Messina, 10Department of Anesthesiology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University, 11Department of Anesthesiology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University This study aims to develop a standard protocol of intra-operative neural monitoring of thyroid surgery in a porcine model. Here, we present a protocol to demonstrate general anesthesia, to compare different types of electrodes, and to investigate the electrophysiological characteristics of the normal and injured recurrent laryngeal nerves. Medicine Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats Hsin-Ting Lin1,2, Yi-Lin Shiou1, Wun-Jyun Jhuang1, Hsiang-Chun Lee1,3,4,5,6 1Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 2Hematology and Oncology, China Medical University Hospital, 3Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, 4Center for Lipid Biosciences, Kaohsiung Medical University Hospital, 5Lipid Science and Aging Research Center, Kaohsiung Medical University, 6Institute/Center of Medical Science and Technology, National Sun Yat-sen University Here, we describe a setup for simultaneous recording of electrocardiography and intra-arterial blood pressure (BP) in experimental rats, which can be done with standard equipment in animal facilities and can be applied to physiological or pharmacological studies to investigate pathogenic or therapeutic mechanisms in cardiovascular medicine. Neuroscience Establishing a Mouse Model of a Pure Small Fiber Neuropathy with the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1 Yi-Chen Lee1,2, Shui-Chin Lu3, Yu-Lin Hsieh1,2 1Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, 2Department of Medical Research, Kaohsiung Medical University Hospital, 3Department of Medical Research, Ultrastructural Laboratory, Kaohsiung Medical University Hospital This study establishes an experimental model of pure small fiber neuropathy with resiniferatoxin (RTX). A unique dose of RTX (50 µg/kg) is optimal for developing a small fiber neuropathy model that mimics patient characteristics and could help investigate the nociceptive molecular significance underlying neuropathic pain. Biology In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity Wan-Shan Yang1, Mel Campbell2, Hsing-Jien Kung2,3,4,5, Pei-Ching Chang1,6 1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University Unlike ubiquitin ligases, few E3 SUMO ligases have been identified. This modified in vitro SUMOylation protocol is able to identify novel SUMO E3 ligases by an in vitro reconstitution assay.
