Huazhong University of Science and Technology

19 articles published in JoVE

• Medicine

  
  Clinical Application of 24 G Cannula Needle and 3-0 Polypropylene Suture in Vas Deferens Exploration Jin Wang*¹, Wenjia Li*², Yao He¹, Yuanbin Xia¹, Lin Sun¹, Youpeng Zhang¹, Zhaohui Zhu^{1 1}Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ²Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology This protocol presents the clinical application of a 24 G cannula and 3-0 polypropylene suture as a simple and effective method for the exploration of the vas deferens.

• Engineering

  
  A Teleoperated Robotic System-Assisted Percutaneous Transiliac-Transsacral Screw Fixation Technique Binbin Liu¹, Fei Xu¹, Yang Liu¹, Tianqi Wang², Yajing Cao², Zehang Zheng¹, Hanqing Xu¹, Chuang Huang¹, Zhengqiang Luo^{1 1}Department of Orthopedics, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, ²Wuhan United Imaging Healthcare Surgical Technology Co. Ltd. Teleoperated robotic system-assisted percutaneous transiliac-transsacral screw fixation is a feasible technique. Screw channels can be implemented with high accuracy owing to the excellent freedom of movement and stability of the robotic arms.

• Biology

  
  A Modified Technique for Transverse Aortic Constriction in Mice Kudusi Abuduwufuer*^1,2, James Jiqi Wang*^1,2, Huihui Li*^1,2, Chen Chen^{1,2 1}Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, ²Hubei Key Laboratory of Genetics and Molecular Mechanism of Cardiological Disorders, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology The present protocol describes a modified and simplified technique with a minimally invasive transverse aortic constriction (TAC) procedure using a self-made retractor. This procedure can be conducted without a ventilator or microscope and introduces pressure overload, eventually leading to cardiac hypertrophy or heart failure.

• Genetics

  
  Assisted Selection of Biomarkers by Linear Discriminant Analysis Effect Size (LEfSe) in Microbiome Data Fang Chang*¹, Shishi He*², Chenyuan Dang^{3 1}Marine Resources Research Centre, Tianjin Research Institute for Water Transport Engineering, M.O.T., ²Key Laboratory of Regional Energy Systems Optimization, Ministry of Education, College of Environmental Science and Engineering, North China Electric Power University, ³School of Environmental Science and Engineering, Huazhong University of Science and Technology LEfSe (LDA Effect Size) is a tool for high-dimensional biomarker mining to identify genomic features (such as genes, pathways, and taxonomies) that significantly characterize two or more groups in microbiome data.

• Neuroscience

  
  A Stably Established Two-Point Injection of Lysophosphatidylcholine-Induced Focal Demyelination Model in Mice Xiao-Wei Pang¹, Man Chen¹, Yun-Hui Chu¹, Yue Tang¹, Chuan Qin¹, Dai-Shi Tian^{1 1}Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology The present protocol describes a two-point injection of lysophosphatidylcholine via a stereotaxic frame to generate a stable and reproducible demyelination model in mice.

• Medicine

  
  Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma Yalan Yu*¹, Hui Shen*¹, Li Liu¹, Ping Luo¹, Sanyun Wu¹, Jing He^1,2, Xiqin Tong¹, Yufeng Shang¹, Liang Shao¹, Fuling Zhou^{1 1}Department of Hematology, Zhongnan Hospital of Wuhan University, ²Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology Here, we present a protocol for improving the success of interphase fluorescence in situ hybridization detection on bone marrow smears from multiple myeloma patients.

• Neuroscience

  
  Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination Hang Zhang¹, Sheng Yang¹, Man Chen¹, Dai-Shi Tian¹, Chuan Qin^{1 1}Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Here, we present a protocol to isolate and purify primary microglia in animal models of demyelinating diseases, utilizing columnar magnetic-activated cell sorting.

• Biology

  
  Assessing Energy Substrate Oxidation In Vitro with ¹⁴CO₂ Trapping Chao Song^1,2, Arianna Valeri¹, Fanxin Long^{1 1} This protocol describes an easy-to-use method to examine substrate oxidation by tracking ¹⁴CO2 production in vitro.

• Neuroscience

  
  Free-floating Immunostaining of Mouse Brains Longlong Tu¹, Nan Zhang^1,2,3, Kristine M Conde¹, Jonathan C Bean¹, Chunmei Wang¹, Yong Xu^{1,4 1}USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, ²Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ³Hubei Provincial Clinical Research Center for Diabetes and Metabolic Disorder, ⁴Department of Molecular and Cellular Biology, Baylor College of Medicine This protocol describes an efficient and reproducible approach for mouse brain histological studies, including perfusion, brain sectioning, free-floating immunostaining, tissue mounting, and imaging.

• Behavior

  
  A Chronic Sleep Fragmentation Model using Vibrating Orbital Rotor to Induce Cognitive Deficit and Anxiety-Like Behavior in Young Wild-Type Mice Yi Xie¹, Sai-Yue Deng¹, Si-Miao Chen¹, Xue-Jiao Chen¹, Wen-Wen Lai¹, Li-Fang Huang¹, Li Ba¹, Wei Wang^1,2, Feng-Fei Ding^{1,3 1}Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China, ²Key Laboratory of Neurological Diseases of Chinese Ministry of Education, The School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000,China, ³Department of Pharmacology, School of Basic Medical Science; State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China Presented here is a protocol for chronic sleep fragmentation (CSF) model achieved by an electrically controlled orbital rotor, which could induce confirmed cognitive deficit and anxiety-like behavior in young wild-type mice. This model can be applied to explore the pathogenesis of chronic sleep disturbance and related disorders.

• Engineering

  
  In Situ Measurement of Vacuum Window Birefringence using ²⁵Mg⁺ Fluorescence W. H. Yuan¹, H. L. Liu¹, W. Z. Wei¹, Z. Y. Ma¹, P. Hao¹, Z. Deng¹, K. Deng¹, J. Zhang¹, Z. H. Lu^{1 1}MOE Key Laboratory of Fundamental Physical Quantities Measurement, Hubei Key Laboratory of Gravitation and Quantum Physics, PGMF and School of Physics, Huazhong University of Science and Technology Presented here is a method to measure the birefringence of vacuum windows by maximizing the fluorescence counts emitted by Doppler cooled ²⁵Mg⁺ ions in an ion trap. The birefringence of vacuum windows will change the polarization states of the laser, which can be compensated by changing the azimuthal angles of external wave plates.

• Medicine

  
  A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice Jie Zhang^1,2, Fang Fang^1,3, Liang Li¹, Haoliang Huang¹, Hannah C. Webber¹, Yang Sun^1,4, Vinit B. Mahajan^1,4, Yang Hu^{1 1}Department of Ophthalmology, Stanford University School of Medicine, ²Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ³Department of Ophthalmology, Second Xiangya Hospital of Central South University, ⁴Department of Ophthalmology, Veterans Affairs Palo Alto Health Care Here, we present a protocol to induce ocular hypertension and glaucomatous neurodegeneration in mouse eyes by intracameral injection of silicone oil and the procedure for silicone oil removal from the anterior chamber to return elevated intraocular pressure to normal.

• Neuroscience

  
  Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5 Shuiqing Chi¹, Mijing Fang¹, Kang Li¹, Li Yang², Shao-tao Tang^{1 1}Department of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ² This protocol describes the process of immunostaining rectal suction biopsies for calretinin, S100 protein, and protein gene product 9.5. This novel adjuvant diagnostic method for Hirschsprung's disease has preferable sensitivity and specificity rates.

• Behavior

  
  Modified Blood Collection from Tail Veins of Non-anesthetized Mice with a Vacuum Blood Collection System and Eyeglass Magnifier Xiaoyan Liu*¹, Huanhuan Li*¹, Jie Wu¹, Yijun Yu¹, Mingjing Zhang¹, Wusong Zou¹, Ye Gu^{1 1}Heart Center, Wuhan Fourth Hospital, Puai Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology This study reports blood sampling from tail vein in mice using a vacuum extraction tube system with eyeglass magnifier. Our method is easy to practice and could be used for repeat blood sampling in mice.

• Engineering

  
  Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro Zilan Xiong*¹, Shasha Zhao*², Xu Yan^{3 1}State Key Laboratory of Advanced Electromagnetic Engineering and Technology, Huazhong University of Science and Technology, ²College of Life Science and Health, Wuhan University of Science and Technology, ³Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University This protocol aims to provide detailed experimental steps of a cold atmospheric plasma treatment on neural stem cells and immunofluorescence detection for differentiation enhancement.

• Bioengineering

  
  Light-sheet Fluorescence Microscopy for the Study of the Murine Heart Yichen Ding¹, Zachary Bailey², Victoria Messerschmidt², Jun Nie³, Richard Bryant², Sandra Rugonyi⁴, Peng Fei³, Juhyun Lee^1,2, Tzung K. Hsiai^{1 1}Department of Bioengineering, University of California Los Angeles, ²Department of Bioengineering, University of Texas at Arlington, ³School of Optical and Electronic Information, Huazhong University of Science and Technology, ⁴Department of Biomedical Engineering, OSHU This study uses a dual-sided illumination light-sheet fluorescence microscopy (LSFM) technique combined with optical clearing to study the murine heart.

• Biochemistry

  
  Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers Huijuan You*¹, Shimin Le*^2,3, Hu Chen^2,4, Linyan Qin¹, Jie Yan^{2,4,5 1}School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, ²Mechanobiology Institute, National University of Singapore, ³Department of Physics, National University of Singapore, ⁴Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Lab for Soft Functional Materials Research, Department of Physics, Xiamen University, ⁵Center for Bioimaging Sciences, National University of Singapore A single-molecule magnetic tweezers platform to manipulate G-quadruplexes is reported, which allows for the study of G4 stability and regulation by various proteins.

• Medicine

  
  Cardiac Catheterization in Mice to Measure the Pressure Volume Relationship: Investigating the Bowditch Effect Bo Zhang^1,2, Jonathan P. Davis¹, Mark T. Ziolo^{1 1}Department of Physiology & Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University, ²Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology This article describes the measurement of murine left ventricular function via pressure/volume analysis at different heart rates.

• Bioengineering

  
  Porous Silicon Microparticles for Delivery of siRNA Therapeutics Jianliang Shen*^1,2, Xiaoyan Wu*^1,3, Yeonju Lee¹, Joy Wolfram^1,4, Zhizhou Yang¹, Zong-Wan Mao², Mauro Ferrari^1,5, Haifa Shen^{1,6 1}Department of Nanomedicine, Houston Methodist Research Institute, ²MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, ³Pediatrics Department of Union Hospital, Huazhong University of Science and Technology, ⁴CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience & Technology of China, ⁵Department of Medicine, Weill Cornell Medical College, ⁶Department of Cell and Developmental Biology, Weill Cornell Medical College Delivery remains the main challenge for the therapeutic implementation of small interfering RNA (siRNA). This protocol involves the use of a multifunctional and biocompatible siRNA delivery platform, consisting of arginine and polyethylenimine grafted porous silicon microparticles.