Kumamoto University

5 articles published in JoVE

• Medicine

  
  Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels Daiki Seya¹, Yuqing Xu^2,3, Toshifumi Mukunoki⁴, Kenichi Tsujita³, Osamu Nakagawa¹, Yuichiro Arima^{1,2,3,4 1}Department of Molecular Physiology, National Cerebral and Cardiovascular Center Research Institute, ²International Research Center for Medical Sciences (IRCMS), Kumamoto University, ³Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, ⁴X-Earth Center, Faculty of Advanced Science and Technology, Kumamoto University Here, we describe a visualization and quantification method for murine hind-limb vessels using micro-X-ray computed tomography.

• Biology

  
  Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate Yen Xuan Ngo^1,2,3, Kenta Haga⁴, Ayako Suzuki⁴, Hiroko Kato⁴, Hiromi Yanagisawa^1,5, Kenji Izumi⁴, Aiko Sada^{1,3 1}Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, ²Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, ³International Research Center for Medical Sciences (IRCMS), Kumamoto University, ⁴Division of Biomimetics, Faculty of Dentistry and Graduate School of Medical and Dental Sciences, Niigata University, ⁵Faculty of Medicine, University of Tsukuba The present protocol describes the isolation and culture of oral keratinocytes derived from the adult mouse palate. An evaluation method using immunostaining is also reported.

• Environment

  
  An Induction System for Clustered Stomata by Sugar Solution Immersion Treatment in Arabidopsis thaliana Seedlings Kae Akita¹, Takumi Higaki^{2 1}Department of Integrated Frontier Sciences, The University of Tokyo, ²International Research Organization for Advanced Science and Technology, Kumamoto University The goal of this protocol is to demonstrate how to induce clustered stomata in cotyledons of Arabidopsis thaliana seedlings by immersion treatment with a sugar-containing medium solution and how to observe intracellular structures such as chloroplasts and microtubules in the clustered guard cells using confocal laser microscopy.

• Neuroscience

  
  In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice Hidenobu Mizuno^1,2,3, Shingo Nakazawa^2,3, Takuji Iwasato^{2,3 1}International Research Center for Medical Sciences (IRCMS), Kumamoto University, ²Division of Neurogenetics, National Institute of Genetics, ³Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies) We present an in vivo two-photon imaging protocol for imaging the cerebral cortex of neonatal mice. This method is suitable for analyzing the developmental dynamics of cortical neurons, the molecular mechanisms that control the neuronal dynamics, and the changes in neuronal dynamics in disease models.

• Bioengineering

  
  Perfusable Vascular Network with a Tissue Model in a Microfluidic Device Yuji Nashimoto¹, Yukako Teraoka¹, Ramin Banan Sadeghian¹, Akiko Nakamasu², Yuichiro Arima³, Sanshiro Hanada³, Hidetoshi Kotera¹, Koichi Nishiyama³, Takashi Miura², Ryuji Yokokawa^{1 1}Department of Micro Engineering, Kyoto University, ²Graduate School of Medical Sciences, Kyushu University, ³International Research Center for Medical Sciences (IRCMS), Kumamoto University The protocol describes how to engineer a perfusable vascular network in a spheroid. The spheroid's surrounding microenvironment is devised to induce angiogenesis and connect the spheroid to the microchannels in a microfluidic device. The method allows the perfusion of the spheroid, which is a long-awaited technique in three-dimensional cultures.