RIKEN Center for Biosystems Dynamics Research

5 articles published in JoVE

• Bioengineering

  
  Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair Takeichiro Nakane^1,2,6, Mosha Abulaiti^1,2, Yuko Sasaki¹, William J. Kowalski³, Bradley B. Keller^4,5,7, Hidetoshi Masumoto^{1,2 1}Clinical Translational Research Program, RIKEN Center for Biosystems Dynamics Research, ²Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, ³Laboratory of Stem Cell and Neuro-Vascular Biology, Cell and Developmental Biology Center, National Institutes of Health, ⁴Kosair Charities Pediatric Heart Research Program, Cardiovascular Innovation Institute, University of Louisville, ⁵Department of Pediatrics, School of Medicine, University of Louisville, ⁶Department of Cardiovascular Surgery, Mitsubishi Kyoto Hospital, ⁷ The present protocol generates mesh-shaped engineered cardiac tissues containing cardiovascular cells derived from human induced pluripotent stem cells to allow the investigation of cell implantation therapy for heart diseases.

• Medicine

  
  A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under Arterial Blood Pressure Hiroomi Nishio^1,3, Kenji Minatoya¹, Hidetoshi Masumoto^{1,2 1}Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, ²Clinical Translational Research Program, RIKEN Center for Biosystems Dynamics Research, ³Department of Cardiovascular Surgery, Takamatsu Red Cross Hospital The present protocol aims to experimentally create venous intimal hyperplasia by subjecting veins to arterial blood pressure for developing strategies to attenuate venous intimal hyperplasia following revascularization surgery using vein grafts.

• Biochemistry

  
  An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects Ahmed Ali^1,2, Yasmine Abouleila^1,2, Arno Germond^{1 1}Biodynamics Research Center (BDR), RIKEN, ²Research Center, Misr International University This protocol presents an integrated Raman spectroscopy-mass spectrometry (MS) platform that is capable of achieving single-cell resolution. Raman spectroscopy can be used to study cellular response to drugs, while MS can be used for targeted and quantitative analysis of drug uptake and metabolism.

• Biochemistry

  
  Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level Simon Leclerc^1,2, Youri Arntz², Yuichi Taniguchi^{1,3 1}Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research, RIKEN, ²Laboratoire de Biomatériaux et Bioimagerie, INSERM, ³PRESTO, Japan Science and Technology Agency Here, we present a protocol to assess the labeling homogeneity for each protein species in a complex protein sample at the single molecule level.

• Biology

  
  TChIP-Seq: Cell-Type-Specific Epigenome Profiling Mari Mito^1,2, Mitsutaka Kadota³, Shinichi Nakagawa^1,4, Shintaro Iwasaki^{2,5 1}RNA Biology Laboratory, RIKEN, ²RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research, ³Laboratory for Phyloinformatics, RIKEN Center for Biosystems Dynamics Research, ⁴RNA Biology Laboratory, Faculty of Pharmaceutical Sciences, Hokkaido University, ⁵Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo We describe a step-by-step protocol for tandem chromatin immunoprecipitation sequencing (tChIP-Seq) that enables the analysis of cell-type-specific genome-wide histone modification.