National Cheng Kung University

10 articles published in JoVE

• Neuroscience

  
  Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice Chia-Wei Liou*^1,2, Tzu-Hsuan Yao*², Wei-Li Wu^{1,2 1}Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, ²Department of Physiology, College of Medicine, National Cheng Kung University Gut-derived microbial metabolites have multifaceted effects leading to complex behavior in animals. We aim to provide a step-by-step method to delineate the effects of gut-derived microbial metabolites in the brain via intracerebroventricular delivery via a guide cannula.

• Medicine

  
  A Mice Model of Chlorhexidine Gluconate-Induced Peritoneal Damage Min-Yu Chang*^1,2, Hsi-Hao Wang*^1,2, Li-Hung Chen*¹, Jhen Gao*¹, Shih-Yuan Hung^1,2, Yuan-Yow Chiou^3,4, Yi-Che Lee^{1,2,5 1}School of Medicine, College of Medicine, I-Shou University, ²Division of Nephrology, Department of Internal Medicine, E-DA Hospital, ³Department of Pediatrics, National Cheng Kung University Hospital, ⁴Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, ⁵Division of Nephrology, Department of Internal Medicine, E-DA Dachang Hospital The present protocol establishes a peritoneal dialysis (PD) mouse model of chlorhexidine gluconate (CG)-induced peritoneal fibrosis. The current model is simple and easy to use compared to other PD animal models.

• Immunology and Infection

  
  Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans Jia-Horung Hung*^1,2, Zhao-Xiang Wang*^3,4, Yuan-Hsin Lo⁵, Chaw-Ning Lee^3,6, Yun Chang⁷, Rita Yuchiao Chang⁷, Chien-Chun Huang³, Tak-Wah Wong^{3,8,9 1}Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, ²Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, ³Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, ⁴Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, ⁵Department of Dermatology, Fu Jen Catholic University Hospital, Fu Jen Catholic University, ⁶Institute of Clinical Pharmacy and Pharmaceutical Sciences, College of Medicine, National Cheng Kung University, ⁷School of Medicine, National Cheng Kung University, ⁸Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, ⁹Center of Applied Nanomedicine, National Cheng Kung University The growing incidence of drug-resistant Candida albicans is a serious health issue worldwide. Antimicrobial photodynamic therapy (aPDT) may offer a strategy to fight against drug-resistant fungal infections. The present protocol describes Rose bengal-mediated aPDT efficacy on a multidrug-resistant C. albicans strain in vitro.

• Immunology and Infection

  
  A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease Ting-Jing Shen^1,2, Ming-Kai Jhan^1,2, Jo-Chi Kao^1,2, Min-Ru Ho^1,2, Tsung-Ting Tsai^1,2, Po-Chun Tseng^1,2, Yung-Ting Wang^1,2, Chiou-Feng Lin^{1,2,3 1}Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, ²Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, ³Center of Infectious Disease and Signaling Research, National Cheng Kung University Here, we present a protocol for creating an immunocompetent ICR (Institute of Cancer Research) murine model of central nervous system infection to display the development of neuropathy. Monitoring acute viral encephalitic disorders by identical disease scores could be performed for showing dengue virus-induced neuropathy in vivo.

• Biology

  
  Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions Yung-Chia Chen^1,2, Bu-Miin Huang^{3 1}Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, ²Department of Anatomy, School of Medicine, Kaohsiung Medical University, ³Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University The hormone secreted by the adrenal cortex is vital for animals against stress and diseases. Here, we present a protocol to culture the primary rat adrenal cells. It could be a good in vitro platform for investigating the mechanisms of the reagent of interest in adrenal steroidogenesis and lipid biosynthesis.

• Immunology and Infection

  
  Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model Yi-Wei Chen¹, Wen-Chien Ko^1,2, Chang-Shi Chen^1,3, Po-Lin Chen^{2,4 1}Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, ²Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, ³Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, ⁴Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University Here, we introduce three different experiments to study Aeromonas infection in C. elegans. Using these convenient methods, it is easy to evaluate the toxicity among and within Aeromonas species.

• Engineering

  
  Uncoupling Coriolis Force and Rotating Buoyancy Effects on Full-Field Heat Transfer Properties of a Rotating Channel Shyy Woei Chang¹, Wei-Ling Cai¹, Hong-Da Shen¹, Kuo-Ching Yu^{1 1}Department of System and Naval Mechatronic Engineering, National Cheng Kung University Here, we present an experimental method for decoupling the interdependent Coriolis-force and rotating-buoyancy effects on full-field heat transfer distributions of a rotating channel.

• Cancer Research

  
  The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues Tsung-Cheng Lin¹, Ying-Chih Liao², Wen-Tsan Chang^1,2, Cheng-Han Yang¹, Li-Hsin Cheng¹, Megan Cheng³, Hung-Chi Cheng^{1,2 1}The Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, ²Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, ³ An animal model is needed to decipher the role of circulating tumor cells (CTCs) in promoting lung colonization during cancer metastasis. Here, we established and successfully performed an in vivo assay to specifically test the requirement of polymeric fibronectin (polyFN) assembly on CTCs for lung colonization.

• Immunology and Infection

  
  Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System Cheng-Ju Kuo^1,2, Sin-Tian Wang^1,2, Chang-Shi Chen^{1,2 1}Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, ²Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University A detailed protocol of a mouse model for enterohemorrhagic E. coli (EHEC) colonization by using bioluminescence-labeled bacteria is presented. The detection of these bioluminescent bacteria by a non-invasive in vivo imaging system in live animals can advance our current understanding of EHEC colonization.

• Behavior

  
  An Innovative Running Wheel-based Mechanism for Improved Rat Training Performance Chi-Chun Chen¹, Chin-Lung Yang², Ching-Ping Chang^{3,4 1}Department of Electronic Engineering, National Chin-Yi University of Technology, ²Department of Electrical Engineering, National Cheng Kung University, ³Department of Biotechnology, Southern Taiwan University of Science and Technology, ⁴Department of Medical Research, Chi Mei Medical Center This study presents an innovative running wheel-based animal mobility system to quantify an effective exercise activity in rats. A rat-friendly testbed is built, using a predefined adaptive acceleration curve, and a high correlation between the effective exercise rate and the infarct volume suggests the protocol's potential for stroke prevention experiments.