3 articles published in JoVE
In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments Sara Nullens1, Annemie Deiteren2, Wen Jiang3, Christopher Keating4, Hannah Ceuleers1, Sven Francque5, David Grundy3, Joris G. De Man1, Benedicte Y. De Winter1 1Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, 2Visceral Pain Group, Discipline of Medicine, University of Adelaide, 3Department of Biomedical Sciences, University of Sheffield, 4Department of Pharmacy, Pharmacology and Postgraduate Medicine, University of Hertfordshire, 5Department of Gastroenterology and Hepatology, Antwerp University Hospital Mesenteric afferent nerves convey information from the gastrointestinal tract towards the brain regarding normal homeostasis as well as pathophysiology. Gastrointestinal afferent nerve activity can be assessed by mounting isolated intestinal segments with attached afferent nerves into an organ bath, isolating the nerve, and assessing basal as well as stimulated activity.
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface Marcia Arenas-Hernandez1, Elly N. Sanchez-Rodriguez1, Tara N. Mial1, Sarah A. Robertson2, Nardhy Gomez-Lopez1,3,4 1Department of Obstetrics & Gynecology, Wayne State University School of Medicine, 2School of Paediatrics and Reproductive Health, Research Centre for Reproductive Health, the Robinson Research Institute, The University of Adelaide, 3Department of Immunology & Microbiology, Wayne State University School of Medicine, 4Perinatology Research Branch, NICHD/NIH/DHHS Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction Avdesh Avdesh*1,2, Mengqi Chen*1,3, Mathew T. Martin-Iverson1,2,4, Alinda Mondal1,3, Daniel Ong1, Stephanie Rainey-Smith1,3, Kevin Taddei1,3, Michael Lardelli5, David M. Groth6, Giuseppe Verdile1,3, Ralph N. Martins1,2,3,7 1Centre of Excellence for Alzheimer's Disease Research and Care, School of Medical sciences, Edith Cowan University, 2Centre for Clinical Research in Neuropsychiatry, Graylands Hospital, University of Western Australia, 3McCusker Alzheimer's Research foundation, 4School of Medicine and Pharmacology, University of Western Australia, 5Department of Molecular and Biomedical Sciences, University of Adelaide, 6School of Biomedical Sciences, Curtin University of Technology, 7School of Psychiatry and Clinical Neurosciences, University of Western Australia This protocol outlines regular maintenance and care to maintain optimal conditions for zebrafish husbandry. The video illustrates the protocol for system maintenance, regular housing, feeding, breeding, and raising of zebrafish larvae.