University of Veterinary Medicine, Vienna View Institution's Website 6 articles published in JoVE Immunology and Infection Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells Fatima Liaqat1, Richard Thiga Kangethe1, Rudolf Pichler1, Bo Liu1, Johann Huber2, Viskam Wijewardana1, Giovanni Cattoli1, Luca Porfiri1 1Animal Production and Health Section, Joint Food and Agriculture Organization (FAO)/International Atomic Energy Agency (IAEA) Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, 2Teaching and Research Farm Kremesberg, Clinical Unit for Herd Health Management in Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna The methodology describes the generation of bovine monocyte-derived dendritic cells (MoDCs) and their application for the in vitro evaluation of antigenic candidates during the development of potential veterinary vaccines in cattle. Biology A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones Ana Marek1, Christiane Schüler1, María Satué1, Barbara Haigl1, Reinhold G. Erben1 1Department of Biomedical Research, University of Veterinary Medicine Vienna A laser capture microdissection (LCM) protocol was developed to obtain sufficient quantity of high-quality RNA for gene expression analysis in bone cells. The current study focusses on mouse femur sections. However, the LCM protocol reported here can be used to study gene expression in cells of any hard tissue. Neuroscience A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins Jürgen Kreiter1, Elena E. Pohl1 1Department of Physiology and Biophysics, University of Veterinary Medicine In electrophysiological measurements, the presence of a diffusion potential disturbs the precise measurement of the reverse potential by altering the electrode potential. Using a micro-agar salt bridge, the impact of the diffusion potential is minimized, which allows a more precise measurement of substrate turnover numbers of reconstituted recombinant membrane proteins. Behavior Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia Katharina Hohlbaum1,2, Bettina Bert2,3, Silke Dietze2, Rupert Palme4, Heidrun Fink2, Christa Thöne-Reineke1 1Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Department of Veterinary Medicine, Freie Universität Berlin, 2Institute of Pharmacology and Toxicology, Department of Veterinary Medicine, Freie Universität Berlin, 3German Federal Institute for Risk Assessment (BfR), 4Unit of Physiology, Pathophysiology and Experimental Endocrinology, Department of Biomedical Sciences, University of Veterinary Medicine We developed a protocol to assess well-being in mice during procedures using general anesthesia. A series of behavioral parameters indicating levels of well-being as well as glucocorticoid metabolites were analyzed. The protocol can serve as a general aid to estimate the degree of severity in a scientific, animal-centered manner. Immunology and Infection Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps Graham Brogden*1,2, Ariane Neumann*1,3, Diab M. Husein1, Friederike Reuner1,4, Hassan Y. Naim1, Maren von Köckritz-Blickwede1,4 1Department of Physiological Chemistry, University of Veterinary Medicine Hannover, 2Fish Disease Research Unit, University of Veterinary Medicine, 3Department of Clinical Sciences, Biomedical Center, Lund University, 4Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover Lipids are known to play an important role in cellular functions. Here, we describe a method to determine the lipid composition of neutrophils, with emphasis on the cholesterol level, by using both HPTLC and HPLC to gain a better understanding of the underlying mechanisms of neutrophil extracellular trap formation. Medicine Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis Xiaoqin Hua1,2, Tobias Deuse1,2, Evangelos D. Michelakis3, Alois Haromy3, Phil S. Tsao4, Lars Maegdefessel4, Reinhold G. Erben5, Claudia Bergow5, Boris B. Behnisch6, Hermann Reichenspurner1,2, Robert C. Robbins7, Sonja Schrepfer1,2,7 1University Heart Center Hamburg, TSI-Lab, Germany, 2Cardiovascular Research Center, University of Hamburg, 3Department of Medicine, Cardiology Division, Pulmonary Hypertension Program, University of Alberta, 4Department of Medicine, Stanford University School of Medicine, 5Department of Biomedical Sciences, Institute of Physiology, Pathophysiology, and Biophysics, University of Veterinary Medicine, Vienna, 6Translumina GmbH, Hechingen, 7Department of Cardiothoracic Surgery, Stanford University School of Medicine This video shows a model to study the development of intimal hyperplasia after stent deployment using a human vessel (IMA) in an immunodeficient rat model.