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Immunology and Infection

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JoVE Journal Immunology and Infection
Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells
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Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

Article DOI: 10.3791/64874-v • 12:54 min • May 19th, 2023
May 19th, 2023 •
1, 1, 1, 1, 2, 1, 1, 1
1Animal Production and Health Section, Joint Food and Agriculture Organization (FAO)/International Atomic Energy Agency (IAEA) Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, 2Teaching and Research Farm Kremesberg, Clinical Unit for Herd Health Management in Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna

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Summary
Automatic Translation

The methodology describes the generation of bovine monocyte-derived dendritic cells (MoDCs) and their application for the in vitro evaluation of antigenic candidates during the development of potential veterinary vaccines in cattle.

Tags

Vaccine Immunogenicity Bovine Monocyte-derived Dendritic Cells In Vitro Assay In Vivo Studies Livestock Vaccinology Dendritic Cells Antigen-presenting Cells Immune Mechanisms Vaccine Efficacy Screening Vaccine Candidates Quality Control Antigen Selection Adjuvant Selection Richard Kangethe Animal Production And Health Laboratory Blood Collection Heparinized Vacutainers Phosphate Buffered Saline (PBS) Lymphocyte Isolation Medium Centrifugation Peripheral Blood Mononuclear Cells (PBMC)
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