Catholic University of Louvain 2 articles published in JoVE Behavior SECONDs Administration Guidelines: A Fast Tool to Assess Consciousness in Brain-injured Patients Leandro R. D. Sanz*1,2, Charlène Aubinet*1,2, Helena Cassol1,2, Olivier Bodart1,2, Sarah Wannez1,2, Estelle A. C. Bonin1,2, Alice Barra1,2, Nicolas Lejeune1,2,3,4, Charlotte Martial1,2, Camille Chatelle1,2, Didier Ledoux5,6, Steven Laureys1,2, Aurore Thibaut1,2, Olivia Gosseries1,2 1Coma Science Group, GIGA-Consciousness, GIGA research center, University of Liège, 2Centre du Cerveau, University Hospital of Liège, 3CHN William Lennox, Groupe Hospitalier Saint-Luc, 4Institute of NeuroScience, UCLouvain, 5Department of Intensive Care, University Hospital of Liège, 6Anesthesia & Intensive Care Laboratory, GIGA-Consciousness, GIGA research center, University of Liège These guidelines can be used for the administration of the Simplified Evaluation of CONsciousness Disorders (SECONDs), a short behavioral tool developed to diagnose brain-injured patients in time-constrained settings. This scale examines command-following, communication, visual pursuit, fixation, pain localization, oriented movements and arousal. Bioengineering The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions Marylène Vandevenne*1, Mathieu Dondelinger*1, Sami Yunus2, Astrid Freischels1, Régine Freischels1, Oscar Crasson1, Noureddine Rhazi1, Pierre Bogaerts3, Moreno Galleni1, Patrice Filée4 1Life Science Department, University of Liège, 2Institute of Condensed Matter and Nanoscience, Catholic University of Louvain, 3Laboratory of Clinical Microbiology, Catholic University of Louvain, 4Biotechnology Department, CER Group In this work, we report a new method to study protein-protein interactions using a conductimetric biosensor based on the hybrid β-lactamase technology. This method relies on release of protons upon hydrolysis of β-lactams.
