Vlaams instituut voor Biotechnologie (VIB) 1 article published in JoVE Biology Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid Larissa Mourao1,2, Marta Ciwinska1,2, Jacco van Rheenen3, Colinda L. G. J. Scheele1,2 1Center for Cancer Biology, VIB (BE), 2Department of Oncology, KU Leuven (BE), 3Netherlands Cancer Institute, Department of Molecular Pathology, Oncode Institute (NL) This protocol describes a novel mammary imaging window with a replaceable lid (R.MIW). Intravital microscopy after implantation of the R.MIW allows for longitudinal and multi-day imaging of the healthy and diseased mammary gland with a cellular resolution during the different developmental stages.
