Leibniz Institute for New Materials (INM) 4 articles published in JoVE Biology Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy Patricia Blach1,2, Sercan Keskin1, Niels de Jonge1,2 1INM-Leibniz Institute for New Materials, 2Department of Physics, Saarland University Presented here is a protocol for labeling membrane proteins in mammalian cells and coating the sample with graphene for liquid-phase scanning transmission electron microscopy. The stability of the samples against the damage caused by radiation can also studied with this protocol. Developmental Biology Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization Silviya Boyadzhieva*1,2, Sarah C.L. Fischer*1,2, Svenja Lösch1,3, Angela Rutz1, Eduard Arzt1,2, Klaus Kruttwig1 1INM - Leibniz Institute for New Materials, 2Department of Materials Science and Engineering, Saarland University, 3University of Applied Sciences Kaiserslautern A protocol for the manufacturing process of polymeric thin film composite structures possessing either different Young's moduli or thicknesses is presented. Films are produced for advanced cell culture studies or as skin adhesives. Engineering Studying Dynamic Processes of Nano-sized Objects in Liquid using Scanning Transmission Electron Microscopy Justus Hermannsdörfer1, Niels de Jonge1,2 1INM-Leibniz Institute for New Materials, 2Department of Physics, University of Saarland This protocol describes the operation of a liquid flow specimen holder for scanning transmission electron microscopy of AuNPs in water, as used for the observation of nanoscale dynamic processes. Bioengineering Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy Diana B. Peckys1, Niels de Jonge1,2 1INM-Leibniz Institute for New Materials, 2Department of Physics, University of Saarland This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. The label contained fluorescent quantum dots. The protocol can be used to study the stoichiometry of EGFR at the single molecule level.
