Boehringer Ingelheim Pharmaceuticals, Inc. 2 articles published in JoVE Biochemistry Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells Vinciane Saint-Criq1, Iram J. Haq2,3, Aaron I. Gardner2, James P. Garnett2,4, Christopher Ward1,2, Malcolm Brodlie2,3, Michael A. Gray1 1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, 3Paediatric Respiratory Medicine, Great North Children's Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co We present a protocol to make dynamic measurements of the airway surface liquid pH under thin film conditions using a plate-reader. Biochemistry Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms Danlin Yang1, Ajit Singh2, Helen Wu1, Rachel Kroe-Barrett1 1Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery, Boehringer Ingelheim Pharmaceuticals, Inc., 2The Fu Foundation School of Engineering and Applied Science, Columbia University We describe here protocols for the measurement of antibody-antigen binding affinity and kinetics using four commonly used biosensor platforms.