Instituto de Investigacion Sanitaria Gregorio Maranon 2 articles published in JoVE Neuroscience In vivo Positron Emission Tomography to Reveal Activity Patterns Induced by Deep Brain Stimulation in Rats Marta Casquero-Veiga1, Nicolás Lamanna-Rama2,3, Diego Romero-Miguel2,3, Manuel Desco1,2,3,4, María Luisa Soto-Montenegro2,4,5 1Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Laboratorio de Imagen Médica, Instituto de Investigación Sanitaria Gregorio Marañón, 3Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 4CIBER de Salud Mental (CIBERSAM), 5High Performance Research Group in Physiopathology and Pharmacology of the Digestive System (NeuGut), Universidad Rey Juan Carlos We describe a preclinical experimental method to evaluate metabolic neuromodulation induced by acute deep brain stimulation with in vivo FDG-PET. This manuscript includes all experimental steps, from stereotaxic surgery to the application of the stimulation treatment and the acquisition, processing, and analysis of PET images. Bioengineering Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform Ignacio Risueño1, Leticia Valencia1, Miguel Holgado2, Jose L. Jorcano3, Diego Velasco1,4 1Department of Bioengineering and Aerospace Engineering, Universidad Carlos III de Madrid (UC3M), 2Group of Optics, Photonics and Biophotonics (GOFB). Center for Biomedical Technology, Universidad Politécnica de Madrid, 3Division of Epithelial Biomedicine, CIEMAT, 4Instituto de Investigación Sanitaria Gregorio Marañón Here, we present a protocol to generate a three-dimensional simplified and undifferentiated skin model using a micromachined microfluidic platform. A parallel flow approach allows the in situ deposition of a dermal compartment for the seeding of epithelial cells on top, all controlled by syringe pumps.
