Newcastle upon Tyne Hospitals NHS Foundation Trust 2 articles published in JoVE Cancer Research A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma Marco Y. W. Zaki*1,2, Shishir Shetty*2, Alex L. Wilkinson2, Daniel A. Patten2, Fiona Oakley*3, Helen Reeves*4,5 1Department of Biochemistry, Faculty of Pharmacy, Minia University, 2National Institute for Health Research Birmingham Liver Biomedical Research Unit and Centre for Liver and Gastrointestinal Research, Institute of Immunology and Immunotherapy, University of Birmingham, 3Newcastle Fibrosis Research Group, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, 4Newcastle University Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, 5The Liver Unit, Department of Medicine, Freeman Hospital, Newcastle-upon-Tyne Hospitals NHS Foundation Trust Comprehensive in vitro models that faithfully recapitulate the relevant human disease are lacking. The current study presents three-dimensional (3D) tumor spheroid creation and culture, a reliable in vitro tool to study the tumor-stromal interaction in human hepatocellular carcinoma. Biochemistry Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells Vinciane Saint-Criq1, Iram J. Haq2,3, Aaron I. Gardner2, James P. Garnett2,4, Christopher Ward1,2, Malcolm Brodlie2,3, Michael A. Gray1 1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, 3Paediatric Respiratory Medicine, Great North Children's Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co We present a protocol to make dynamic measurements of the airway surface liquid pH under thin film conditions using a plate-reader.
