Radboud University Medical Center 13 articles published in JoVE Medicine Precision of In Vivo Quantitative Tooth Wear Measurement Using Intra-Oral Scans Hilde Bronkhorst1, Ewald Bronkhorst1, Stanimira Kalaykova1, Wicher van der Meer2, Marie-Charlotte Huysmans1, Bas Loomans1 1Department of Dentistry, Radboud University Medical Center, 2Department of Orthodontics, University Medical Center Groningen, University of Groningen Quantitative wear measurement is a method of increasing importance in measuring tooth wear progression. We here describe a protocol, its precision, and its intra/inter-rater precision for the acquisition and superimposition of repeated in vivo scanned dentitions in patients with moderate to severe wear, reporting on both height and volume measurements. Neuroscience Maximum Isometric Tetanic Force Measurement of the Tibialis Anterior Muscle in the Rat Meiwand Bedar1,2, Tiam M. Saffari1,2, Patricia F. Friedrich1, Guilherme Giusti1, Allen T. Bishop1, Alexander Y. Shin1 1Department of Orthopedic Surgery, Mayo Clinic, 2Radboud University Medical Center, Radboud Institute for Health Sciences, Department of Plastic Surgery, Nijmegen, The Netherlands Evaluation of motor recovery remains the benchmark outcome measure in experimental peripheral nerve studies. The isometric tetanic force measurement of the tibialis anterior muscle in the rat is an invaluable tool to assess functional outcomes after reconstruction of sciatic nerve defects. The methods and nuances are detailed in this article. Biology Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation Jennifer Eymael1, Laura Miesen1, Fieke Mooren1, Jitske Jansen1,2, Jack Wetzels3, Johan van der Vlag4, Bart Smeets1 1Radboud Institute for Molecular Life Sciences, Department of Pathology, Radboud University Medical Center, 2Radboud Institute for Molecular Life Sciences, Amalia Children's Hospital, Department of Paediatric Nephrology, Radboud University Medical Center, 3Radboud Institute for Health Sciences, Department of Nephrology, Radboud University Medical Center, 4Radboud Institute for Molecular Life Sciences, Department of Nephrology, Radboud University Medical Center This article describes a method for culturing and analyzing glomerular parietal epithelial cell outgrowths of encapsulated glomeruli isolated from mouse kidney. This method can be used to study pathways involved in parietal epithelial cell proliferation and migration. Genetics Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis Jos G.A. Smits*1, Jieqiong Qu*1, Hanna Niehues2, Huiqing Zhou1,3 1Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands, 2Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, 3Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands Presented here is a stepwise procedure for in vitro differentiation of human primary keratinocytes by contact inhibition followed by characterization at the molecular level by RNA-seq analysis. Cancer Research E-Patient Counseling Trial (E-PACO): Computer Based Education versus Nurse Counseling for Patients to Prepare for Colonoscopy Govert Veldhuijzen1, Aura A. van Esch1, Michael Klemt-Kropp2, Jochim S. Terhaar sive Droste3, Joost P.H. Drenth1 1Department of Gastroenterology and Hepatology, Radboud University Medical Center, 2Department of Gastroenterology and Hepatology, North West Clinics, 3Department of Gastroenterology and Hepatology, Jeroen Bosch Hospital The aim of this trial is to establish the position of online computer-based education as a tool for patient preparation prior to a colonoscopy. Computer based education is compared with the standard of care, nurse counseling, evaluating endoscopic quality measures and patient related outcome measures. Medicine The Dyspepsia Educational Tool As a Novel Aid in Dyspepsia Management Judith J. de Jong1, Marten A. Lantinga1, Joost P.H. Drenth1 1Department of Gastroenterology and Hepatology, Radboud University Medical Center This protocol describes the development process of a digital dyspepsia educational tool. Assessment of unmet needs and literature, content development, and building of the tool are presented. The methodology can be used as a guide for future development of digital educational tools. Neuroscience Two Algorithms for High-throughput and Multi-parametric Quantification of Drosophila Neuromuscular Junction Morphology Anna Castells-Nobau*1, Bonnie Nijhof*1, Ilse Eidhof1, Louis Wolf2, Jolanda M. Scheffer-de Gooyert1, Ignacio Monedero3,4, Laura Torroja3, Jeroen A.W.M. van der Laak2,5, Annette Schenck*1 1Department of Human Genetics, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, 2Microscopical Imaging Centre (MIC), Radboud University Medical Center, 3Department of Biology, Universidad Autónoma de Madrid, 4Department of Clinical and Experimental Medicine, Linköping University, 5Department of Pathology, Radboud University Medical Center Two image analysis algorithms, "Drosophila NMJ Morphometrics" and "Drosophila NMJ Bouton Morphometrics" were created, to automatically quantify nine morphological features of the Drosophila neuromuscular junction (NMJ). Developmental Biology Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays Monica Frega*1,2, Sebastianus H. C. van Gestel*3, Katrin Linda2,3, Jori van der Raadt3, Jason Keller1,2, Jon-Ruben Van Rhijn1,2, Dirk Schubert1,2, Cornelis A. Albers2,3,4, Nael Nadif Kasri1,2,3 1Department of Cognitive Neurosciences, Radboudumc, 2Donders Institute for Brain, Cognition and Behaviour, Radboud University, 3Department of Human Genetics, Radboudumc, 4Department of Molecular Developmental Biology, Radboud University We modify and implement a previously published protocol describing the rapid, reproducible, and efficient differentiation of human induced Pluripotent Stem Cells (hiPSCs) into excitatory cortical neurons12. Specifically, our modification allows for control of neuronal cell density and use on micro-electrode arrays to measure electrophysiological properties at the network level. Neuroscience Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model Mariateresa Tedesco1, Monica Frega1,2, Sergio Martinoia1, Mattia Pesce3, Paolo Massobrio1 1Department of Informatics, Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, 2Donders Institute for Brain, Cognition and Behaviour, Department of Cognitive Neuroscience, Radboud University Medical Center, 3Fondazione Istituto Italiano di Tecnologia (IIT) In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures. Medicine Using an Ingestible Telemetric Temperature Pill to Assess Gastrointestinal Temperature During Exercise Coen C.W.G. Bongers1, Maria T.E. Hopman1, Thijs M.H. Eijsvogels1 1Radboud Institute for Health Sciences, Department of Physiology, Radboud University Medical Center This study describes an accurate, reliable and non-invasive technique to continuously measure gastrointestinal temperature during exercise. The ingestible telemetric temperature pill is suitable to measure gastrointestinal temperature in laboratory settings as well as in field based settings. Developmental Biology Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles Paola L. Carvajal Monroy1, Zipora Yablonka-Reuveni2, Sander Grefte3, Anne Marie Kuijpers-Jagtman1, Frank A.D.T.G. Wagener1, Johannes W. Von den Hoff1 1Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 2Department of Biological Structure, University of Washington School of Medicine, 3Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center This protocol describes the isolation of satellite cells from branchiomeric head muscles of a 9 week-old rat. The muscles originate from different branchial arches. Subsequently, the satellite cells are cultured on a spot coating of millimeter size to study their differentiation. This approach avoids the expansion and passaging of satellite cells. Immunology and Infection An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection Marloes Vissers1, Marrit N. Habets1, Inge M. L. Ahout1, Jop Jans1, Marien I. de Jonge1, Dimitri A. Diavatopoulos1, Gerben Ferwerda1 1Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, Radboud university medical center Human respiratory syncytial virus (HRSV) can cause severe bronchiolitis in young infants. Part of the pathogenesis of severe HRSV disease is caused by the host immune response. Stimulation of primary human immune cells with HRSV provides a fast and reproducible model system to study activation of inflammatory pathways and infection. Medicine In vivo 19F MRI for Cell Tracking Mangala Srinivas1, Philipp Boehm-Sturm2, Markus Aswendt2, Eberhard D. Pracht2,3, Carl G. Figdor1, I. Jolanda de Vries1, Mathias Hoehn2 1Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, Radboud University Medical Center, 2In-Vivo-NMR Laboratory, Max Planck Institute for Neurological Research, 3German Center for Neurodegenerative Diseases (DZNE) We describe a general protocol for in vivo cell tracking using MRI in a mouse model with ex vivo labeled cells. A typical protocol for cell labeling, image acquisition processing and quantification is included.
