Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Glomerular Outgrowth as an Ex Vivo Assay to…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
Journal (Biology)
Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
Journal (Biology)
The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
 

Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

Article DOI: 10.3791/60324-v • 06:39 min • August 19th, 2020
August 19th, 2020 •
1, 1, 1, 1,2, 3, 4, 1
1Radboud Institute for Molecular Life Sciences, Department of Pathology, Radboud University Medical Center, 2Radboud Institute for Molecular Life Sciences, Amalia Children's Hospital, Department of Paediatric Nephrology, Radboud University Medical Center, 3Radboud Institute for Health Sciences, Department of Nephrology, Radboud University Medical Center, 4Radboud Institute for Molecular Life Sciences, Department of Nephrology, Radboud University Medical Center

Chapters

Summary
Automatic Translation

This article describes a method for culturing and analyzing glomerular parietal epithelial cell outgrowths of encapsulated glomeruli isolated from mouse kidney. This method can be used to study pathways involved in parietal epithelial cell proliferation and migration.

Tags

Glomerular Outgrowth Ex Vivo Assay Pathways Parietal Epithelial Cell Activation Scar Tissue Development Kidney Glomerulus Cellular Processes Treatment Options Primary Cells Isolated Glomeruli Surgical Forceps Renal Capsules Culture Plate HBSS Petri Dish Scalpels Minced Kidney Pieces Metal Sieve Plunger Syringe Flow-through Serological Pipette Kidney Homogenate
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter