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4 articles published in JoVE
Cystometric and External Urethral Sphincter Measurements in Awake Rats with Implanted Catheter and Electrodes Allowing for Repeated Measurements Elena E. Foditsch1,2, Karin Roider1,2, Andrea M. Sartori3,4,5, Thomas M. Kessler5, Sabik Raj Kayastha6, Ludwig Aigner2, Marc P. Schneider7 1Department of Urology, Spinal Cord Injury and Tissue Regeneration Center Salzburg, Paracelsus Medical University, 2Institute of Molecular Regenerative Medicine, Spinal Cord Injury and Tissue Regeneration Center Salzburg, Paracelsus Medical University, 3Brain Research Institute, University of Zürich, 4Department of Health Sciences and Technology, Swiss Federal Institute of Technology Zürich, 5Neuro-Urology, Spinal Cord Injury Center & Research, University of Zürich, Balgrist University Hospital, 6Department of Orthopaedics and Traumatology, Dhulikhel Hospital, Kathmandu University Hospital, 7Department of Urology, Inselspital, Bern University Hospital This protocol first describes the surgical procedure of the permanent implantation of a urinary bladder catheter combined with external urethral sphincter electrodes, and second, the measurement of the function of the urinary bladder and external urethral sphincter in implanted awake animals.
Easy and Accurate Mechano-profiling on Micropost Arrays Nils Goedecke1, Maja Bollhalder1, Remo Bernet1, Unai Silvan1, Jess Snedeker1,2 1University of Zurich, Balgrist University Hospital, 2Institute for Biomechanics, ETH Zurich Described are protocols for quantifying mechanical interactions between adherent cells and microstructured substrates. These interactions are closely linked to essential cell behaviors including migration, proliferation, differentiation, and apoptosis. The protocols present an open-source image analysis software called MechProfiler, which enables determination of involved forces for each micropost.
Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro Daniela Meier*1, Carmen Campanile*1, Sander M. Botter1, Walter Born1, Bruno Fuchs1 1Laboratory for Orthopedic Research, Balgrist University Hospital, Zurich, Switzerland The protocol reported here allows the evaluation of the efficacy of photodynamic therapy (PDT) in cell lines and the optimization of the PDT settings before applying the therapy in animal models.
Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells Matthias J.E. Arlt1, Walter Born1, Bruno Fuchs1 1Laboratory for Orthopedic Research, Balgrist University Hospital, Zurich The novel protocol reported in the present study allows selective detection of lung metastases at single cell resolution in mice by combined in-situ lung perfusion and fixation and X-Gal staining of lacZ-tagged tumor cells.