3 articles published in JoVE
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer Susan Kochall1, May-Linn Thepkaysone1, Sebastián A. García1, Alexander M. Betzler1, Jürgen Weitz1,2,3, Christoph Reissfelder1, Sebastian Schölch1,2,3 1Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, 2German Cancer Consortium (DKTK), 3German Cancer Research Center (DKFZ) We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.
Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound Tobias Bäuerle1, Dorde Komljenovic1, Martin R. Berger2, Wolfhard Semmler1 1Department of Medical Physics in Radiology, German Cancer Research Center, Heidelberg, Germany, 2Unit of Chemotherapy and Toxicology, German Cancer Research Center, Heidelberg, Germany In the pathogenesis of bone metastasis, angiogenesis is a crucial process and therefore represents a target for imaging and therapy. Here, we present a rat model of site-specific breast cancer bone metastasis and describe strategies to non-invasively image angiogenesis in vivo using magnetic resonance imaging, volumetric computed tomography and ultrasound.
Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems Nazim El-Andaloussi*1,2, Barbara Leuchs*1, Serena Bonifati1,2, Jean Rommelaere1,2, Antonio Marchini1,2 1Tumour Virology Division F010, German Cancer Research Center (DKFZ), 2Inserm Unit 701, German Cancer Research Center (DKFZ) Here we describe a protocol based only on cell infection, which improves the efficiency of recombinant parvovirus production by more than 100 fold in comparison to other protocols in use. This protocol relies on the use of a novel adenovirus 5-based helper containing the parvovirus VP transcription unit (Ad-VP).