2 articles published in JoVE
An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers Kavi Devraj1,2, Sylvaine Guérit1, Jakranka Macas1, Yvonne Reiss1 1Institute of Neurology (Edinger Institute), Goethe University Hospital, 2Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology, Goethe University Hospital Here we present a mouse brain vascular permeability assay using intraperitoneal injection of fluorescent tracers followed by perfusion that is applicable to animal models of blood-brain barrier dysfunction. One hemi-brain is used for assessing permeability quantitatively and the other for tracer visualization/immunostaining. The procedure takes 5 - 6 h for 10 mice.
Bioluminescence and Near-infrared Imaging of Optic Neuritis and Brain Inflammation in the EAE Model of Multiple Sclerosis in Mice Katja Schmitz1, Irmgard Tegeder1 1Institute of Clinical Pharmacology, University Hospital Frankfurt We show a technique for in vivo live bioluminescence and near-infrared imaging of optic neuritis and encephalitis in the experimental autoimmune encephalomyelitis (EAE) model for multiple sclerosis in SJL/J mice.