LABEO Frank Duncombe 1 article published in JoVE Immunology and Infection Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids Erika S. Hue1,2,3, Christine I. Fortier1,3, Aurélie M. Laurent1, Yann F. Quesnelle1, Guillaume D. Fortier1,2,3, Loïc J. Legrand1,2,3, Stephane L. Pronost1,2,3 1LABÉO Frank Duncombe, 2Unité de Risques Microbiens (U2RM, EA 4655), Normandy University, 3Hippolia Foundation Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.
