1 article published in JoVE
The MultiBac Protein Complex Production Platform at the EMBL Imre Berger1, Frederic Garzoni1, Maxime Chaillet1, Matthias Haffke1, Kapil Gupta1, Alice Aubert1 1EMBL Grenoble Outstation and Unit of Virus Host Cell Interactions (UVHCI) UMR5322 Protein complexes catalyze key cellular functions. Detailed functional and structural characterization of many essential complexes requires recombinant production. MultiBac is a baculovirus/insect cell system particularly tailored for expressing eukaryotic proteins and their complexes. MultiBac was implemented as an open-access platform, and standard operating procedures developed to maximize its utility.