1 article published in JoVE

• Neuroscience

  
  In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments Sara Nullens¹, Annemie Deiteren², Wen Jiang³, Christopher Keating⁴, Hannah Ceuleers¹, Sven Francque⁵, David Grundy³, Joris G. De Man¹, Benedicte Y. De Winter^{1 1}Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, ²Visceral Pain Group, Discipline of Medicine, University of Adelaide, ³Department of Biomedical Sciences, University of Sheffield, ⁴Department of Pharmacy, Pharmacology and Postgraduate Medicine, University of Hertfordshire, ⁵Department of Gastroenterology and Hepatology, Antwerp University Hospital Mesenteric afferent nerves convey information from the gastrointestinal tract towards the brain regarding normal homeostasis as well as pathophysiology. Gastrointestinal afferent nerve activity can be assessed by mounting isolated intestinal segments with attached afferent nerves into an organ bath, isolating the nerve, and assessing basal as well as stimulated activity.