University of Hertfordshire

2 articles published in JoVE

• Biology

  
  Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells Alexander Hamilton^1,2, Elisa Vergari¹, Caroline Miranda³, Andrei I. Tarasov^{1,4,5 1}Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, ²Lund University Diabetes Centre, Unit of Molecular Metabolism, Clinical Research Centre, Malmö University HospitalMalmö, ³Institute of Neuroscience of Physiology, Department of Physiology, Metabolic Research Unit, University of Göteborg, ⁴Oxford National Institute for Health Research, Biomedical Research Centre, ⁵Life and Medical Sciences, University of Hertfordshire Here, we present a protocol for imaging and quantifying calcium dynamics in heterogeneous cell populations, such as pancreatic islet cells. Fluorescent reporters are delivered into the peripheral layer of cells within the islet, which is then immobilized and imaged, and per-cell analysis of the dynamics of fluorescence intensity is performed.

• Neuroscience

  
  In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments Sara Nullens¹, Annemie Deiteren², Wen Jiang³, Christopher Keating⁴, Hannah Ceuleers¹, Sven Francque⁵, David Grundy³, Joris G. De Man¹, Benedicte Y. De Winter^{1 1}Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, ²Visceral Pain Group, Discipline of Medicine, University of Adelaide, ³Department of Biomedical Sciences, University of Sheffield, ⁴Department of Pharmacy, Pharmacology and Postgraduate Medicine, University of Hertfordshire, ⁵Department of Gastroenterology and Hepatology, Antwerp University Hospital Mesenteric afferent nerves convey information from the gastrointestinal tract towards the brain regarding normal homeostasis as well as pathophysiology. Gastrointestinal afferent nerve activity can be assessed by mounting isolated intestinal segments with attached afferent nerves into an organ bath, isolating the nerve, and assessing basal as well as stimulated activity.