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Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci
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Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci

Article DOI: 10.3791/62017-v • 09:07 min • March 4th, 2021
March 4th, 2021 •
1, 2, 1, 2
1Laboratory of Nuclear and Genomic Health, Centre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Brunel University London, 2Biosciences, Department of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire

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Summary
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Combining DNA halo preparations with fluorescence in situ hybridization enables high-resolution analysis of genomic interactions with the nucleoskeleton. Attached genome leads to hybridized fluorescent signals located within the residual extracted nuclei, whereas non-attached genome is in the halo of DNA surrounding the residual nuclei.

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Fluorescence In Situ Hybridization DNA Halo Preparations Whole Chromosomes Telomeres Gene Loci Nucleoskeleton Attachments DamID Sequencing Single Cell Assay Functional Differences Visualization Techniques Spatial Organization Functional Organization Treated Cancer Cells Progeria Cells Senescent Cells Genomic Behavioral Differences Model Organisms Probes Hydrochloric Acid Microscope Slides Tap Water Deionized Water Methanol Sterilization Autoclave Hot Oven Cell Growth
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