2 articles published in JoVE
Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells Balta Lucendo-Villarin1, Hassan Rashidi1,2, Sharmin Alhaque1,3, Lena Fischer1,4, Jose Meseguer-Ripolles1, Yu Wang1, Cliona O'Farrelly4, Michael Themis3, David C. Hay1 1MRC Centre for Regenerative Medicine, University of Edinburgh, 2UCL Great Ormond Street Institute of Child Health, University College London, 3Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences, Brunel University London, 4School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin This protocol describes an approach to produce hepatospheres from human pluripotent stem cells using a defined culture system and cell self-assembly. This protocol is reproducible in a number of cell lines, cost effective and allows the production of stable human hepatospheres for biomedical application.
Use of a Battery of Chemical and Ecotoxicological Methods for the Assessment of the Efficacy of Wastewater Treatment Processes to Remove Estrogenic Potency Nicola Beresford1, Alice Baynes1, Rakesh Kanda1, Matthew R. Mills2, Karla Arias-Salazar2, Terrence J. Collins2, Susan Jobling1 1Institute of Environment Health and Societies, Brunel University London, 2Department of Chemistry, Carnegie Mellon University Endocrine Disrupting Compounds (EDC) pose a substantial risk to the aquatic environment. Municipal wastewater treatment plants are major contributors to the estrogenic potency of surface waters. The methodology provided in this paper allows for an assessment of the efficacy and suitability of wastewater treatment processes with respect to EDC removal.