3 articles published in JoVE
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors Rita Silvério-Alves1,2,3, Andreia M. Gomes3, Ilia Kurochkin4, Kateri A. Moore5,6, Carlos-Filipe Pereira1,2,3 1Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, 2Wallenberg Center for Molecular, Lund University, 3Center for Neuroscience and Cell Biology, University of Coimbra, 4Skolkovo Institute of Science and Technology, 5Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 6Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai This protocol demonstrates the induction of a hemogenic program in human dermal fibroblasts by enforced expression of the transcription factors GATA2, GFI1B and FOS to generate hematopoietic stem and progenitor cells.
Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization Xavier Morató*1,2, Marc López-Cano*1,2, Paula M. Canas3, Rodrigo A. Cunha3, Francisco Ciruela1,2 1Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL, Universitat de Barcelona, L'Hospitalet de Llobregat, 2Institut de Neurociències, Universitat de Barcelona, 3Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine, University of Coimbra Here, we present a brain membrane fractionation protocol that represents a robust procedure to isolate proteins belonging to different synaptic compartments.
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program Michael G. Daniel1,2,3, Carlos-Filipe Pereira4, Jeffrey M. Bernitz1,2,3, Ihor R. Lemischka1,3,5, Kateri Moore1,3 1Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 2The Graduate School of Biomedical Science, Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 4Center for Neuroscience and Cell Biology, University of Coimbra, 5Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai The protocol described here details the induction of a hemogenic program in mouse embryonic fibroblasts via overexpression of a minimal set of transcription factors. This technology may be translated to the human system to provide platforms for future study of hematopoiesis, hematologic disease, and hematopoietic stem cell transplant.