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Accessing the Cytotoxicity and Cell Response to Biomaterials
JoVE Journal
Medicine
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JoVE Journal Medicine
Accessing the Cytotoxicity and Cell Response to Biomaterials
DOI:

09:46 min

July 08, 2021

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Chapters

  • 00:05Introduction
  • 01:12Pellet Preparation and Soluble Biomaterial Extracts Acquisition
  • 02:11Morphology Evaluation
  • 03:28Cell Function Assessment
  • 05:54Mineralized Formation Assessment
  • 06:50Results: Cell Viability, Morphology, and Protein Expression in Response to Biomaterial Exposure
  • 08:30Conclusion

Summary

Automatic Translation

This methodology aims to evaluate biomaterial cytotoxicity through the preparation of soluble extracts, using viability assays and phenotypic analysis, including flow cytometry, RT-PCR, immunocytochemistry, and other cellular and molecular biology techniques.

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