H. Lee Moffitt Cancer Center and Research Institute 4 articles published in JoVE Cancer Research Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product Ana Marie Landin1, Cheryl Cox1, Bin Yu2, Nelli Bejanyan1,2, Marco Davila1,2, Linda Kelley1,2 1Cell Therapy Facility, Moffitt Cancer Center and Research Institute, 2Department of Immunology, Moffitt Cancer Center and Research Institute Presented is a protocol for the expansion of Gamma-Delta (γδ) T cell drug product. Lymphocytes are isolated by elutriation and γδ-enriched with zoledronic acid and interleukin-2. Alpha-beta T cells are depleted using a clinical-grade magnetic separation device. The γδ cells are co-cultured with K562-derived, artificial antigen-presenting cells and expanded. Cancer Research A Murine Ommaya Xenograft Model to Study Direct-Targeted Therapy of Leptomeningeal Disease Vincent Law1,2, Margi Baldwin3, Ganesan Ramamoorthi4, Krithika Kodumudi4, Nam Tran1, Inna Smalley5, Derek Duckett6, Pawel Kalinski7, Brian Czerniecki4, Keiran S. M. Smalley2, Peter A. Forsyth1,2 1Department of Neuro-Oncology, H. Lee Moffitt Cancer Center & Research Institute, 2Department of Tumor Biology, H. Lee Moffitt Cancer Center & Research Institute, 3Department of Comparative Medicine, University of South Florida, 4Department of Breast Oncology, H. Lee Moffitt Cancer Center & Research Institute, 5Department of Cancer Physiology, H. Lee Moffitt Cancer Center & Research Institute, 6Department of Drug Discovery, H. Lee Moffitt Cancer Center & Research Institute, 7Department of Medical Oncology, Roswell Park Comprehensive Cancer Center Here, we describe a murine xenograft model that functionally resembles an Ommaya reservoir in patients. We developed the Murine Ommaya to study novel therapeutics for the universally fatal leptomeningeal disease. Medicine Cardiac Magnetic Resonance for the Evaluation of Suspected Cardiac Thrombus: Conventional and Emerging Techniques Elizabeth M. Johnson1, Kenneth L. Gage2, Sebastian Feuerlein2, Daniel Jeong2 1Department of Radiology, University of South Florida, 2Department of Diagnostic Imaging, H. Lee Moffitt Cancer Center and Research Institute The goal of this article is to describe how cardiac magnetic resonance can be used for the evaluation and diagnosis of a suspected cardiac thrombus. The method presented will describe data acquisition as well as the pre-procedure and post-procedure protocol. Medicine An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells Ariosto Silva1, Timothy Jacobson1, Mark Meads1, Allison Distler1, Kenneth Shain1 1Department of Cancer Imaging and Metabolism, H. Lee Moffitt Cancer Center and Research Institute We describe a high-throughput drug sensitivity assay for primary multiple myeloma cells. It consists of a reconstruction of the bone marrow microenvironment (including extracellular matrix and stroma) in multi-well plates, and a non-invasive method for longitudinal quantification of cell viability.
