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Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

Article DOI: 10.3791/62622-v • 11:51 min • September 22nd, 2021
September 22nd, 2021 •
1, 1, 2, 1,2, 1,2, 1,2
1Cell Therapy Facility, Moffitt Cancer Center and Research Institute, 2Department of Immunology, Moffitt Cancer Center and Research Institute

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Summary
Automatic Translation

Presented is a protocol for the expansion of Gamma-Delta (γδ) T cell drug product. Lymphocytes are isolated by elutriation and γδ-enriched with zoledronic acid and interleukin-2. Alpha-beta T cells are depleted using a clinical-grade magnetic separation device. The γδ cells are co-cultured with K562-derived, artificial antigen-presenting cells and expanded.

Tags

Gamma-Delta T Cells Cytotoxic Activity Cancer Acute Myeloid Leukemia Expansion Enrichment Apheresed Human Product X-vivo Expanded Gamma-Delta T Cells Recurrence Of Leukemia Survival Therapeutically Relevant Doses Highly Pure Gamma-Delta T Cell Product Ligating An IEL Alpha Beta T Cell Depletion Magnetic Sorting Secondary Expansion With AAPCs Utility Of Gamma-Delta T Cells Off The Shelf Cancer Autoimmune Diseases Enrichment Methods Transduction Elutriation Counterflow Centrifugation Device
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