Beth Israel Deaconess Medical Center & Harvard Medical School 7 articles published in JoVE Cancer Research The Clinical Application of Tumor Treating Fields Therapy in Glioblastoma Mercedes M. Riley1,2, Pyay San1, Edwin Lok1, Kenneth D. Swanson1, Eric T. Wong1 1Brain Tumor Center and Neuro-Oncology Unit, Department of Neurology, Harvard Medical School, Beth Israel Deaconess Medical Center, 2Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology Glioblastoma is the most common and aggressive primary brain malignancy in adults, with most tumors recurring after initial treatment. Tumor Treating Fields (TTFields) therapy is the newest treatment modality for glioblastoma. Here, we describe the proper application of TTFields-transducer arrays on patients and discuss theory and aspects of treatment. Behavior Mimicking a Space Mission to Mars Using Hindlimb Unloading and Partial Weight Bearing in Rats Marie Mortreux1, Daniela Riveros1, Mary L. Bouxsein2, Seward B. Rutkove1 1Department of Neurology, Harvard Medical School - Beth Israel Deaconess Medical Center, 2Department of Orthopaedics, Harvard Medical School - Beth Israel Deaconess Medical Center By using an innovative ground-based analogue model, we are able to simulate a space mission including a trip to (0 g) and a stay on Mars (0.38 g) in rats. This model allows for a longitudinal assessment of the physiological changes occurring during the two hypo-gravitational stages of the mission. Medicine Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT Katherine Kim1, Shan Huang2, Laura A. Fletcher1, Alana E. O'Mara1, Ilan Tal3, Robert J. Brychta1, Aaron M. Cypess1, Kong Y. Chen1, Brooks P. Leitner1 1Diabetes, Endocrinology, and Obesity Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 2National Cancer Institute, National Institutes of Health, 3Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School Using free, open-source software, we have developed an analytical approach to quantify total and regional brown adipose tissue (BAT) volume and metabolic activity of BAT using 18F-FDG PET/CT. Bioengineering Rat Model of Adhesive Capsulitis of the Shoulder Stephen M. Okajima*1, M. Belen Cubria*1, Sharri J. Mortensen1, Juan C. Villa-Camacho1, Philip Hanna1, Aron Lechtig1, Miguel Perez-Viloria1, Patrick Williamson1, Mark W. Grinstaff*3, Edward K. Rodriguez*2, Ara Nazarian*1,4 1Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center and Harvard Medical School, 2Carl J. Shapiro Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, 3Departments of Biomedical Engineering, Chemistry, and Medicine, Boston University, 4Department of Orthopaedic Surgery, Yerevan State Medical University This protocol presents an in vivo rat model of adhesive capsulitis. The model includes an internal fixation of the glenohumeral joint with extra-articular suture fixation for an extended time, resulting in a decreased rotational range of motion (ROM) and increased joint stiffness. Medicine Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs Joshua J. Timmons1, Sean Cohessy2, Eric T. Wong1 1Brain Tumor Center & Neuro-Oncology Unit, Department of Neurology, Beth Israel Deaconess Medical Center & Harvard Medical School, 2Division of Cancer Genetics, Department of Medicine, Beth Israel Deaconess Medical Center & Harvard Medical School Metastasis plays a profound role in the virulence of cancer, accounting for an estimated 90% of deaths. We report a protocol for a metastatic melanoma model in mice that is useful for determining the efficacy of therapeutic agents against this clinical phenomenon. Biology Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration Janine M. Preble1, Christina A. Pacak2, Hiroshi Kondo1, Allison A. MacKay2, Douglas B. Cowan2, James D. McCully1 1Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, 2 A method for rapid isolation of mitochondria from mammalian tissue biopsies is described. Rat liver or skeletal muscle preparations were homogenized with a commercial tissue dissociator and mitochondria were isolated by differential filtration through nylon mesh filters. Mitochondrial isolation time is <30 min compared to 60 - 100 min using alternative methods. Biology Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes Evan Lee Graham1, Cristina Balla1,2, Hannabeth Franchino1, Yonathan Melman1, Federica del Monte*1, Saumya Das*1 1Cardiovascular Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Division of Cardiology, Sapienza University Here we describe the isolation of adult mouse cardiomyoctyes using a Langendorff perfusion system. The resulting cells are Ca2+-tolerant, electrically quiescent and can be cultured and transfected with adeno- or lentiviruses to manipulate gene expression. Their functionality can also be analyzed using the MMSYS system and patch clamp techniques.
