Rowan University 4 articles published in JoVE Genetics Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response Stephen D. Willis1, A. K. M. Nawshad Hossian2, Nathan Evans2, Mark J. Hickman2 1Department of Molecular Biology, Rowan School of Osteopathic Medicine, 2Department of Biological Sciences, Rowan University Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular response. Bioengineering Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering Thomas R. Christiani1, Katelynn Toomer2, Joseph Sheehan2, Angelika Nitzl2, Amanda Branda2, Elizabeth England2, Pamela Graney3, Cristina Iftode2, Andrea J. Vernengo1 1Department of Chemical Engineering, Rowan University, 2Department of Biological Sciences, Rowan University, 3Department of Biomedical Engineering, Drexel University An injectable tissue engineering scaffold composed of poly(N-isopropylacrylamide)-graft-chondroitin sulfate (PNIPAAm-g-CS)-containing alginate microparticles was prepared. The adhesive strength, swelling properties and in vitro biocompatibility are analyzed in this study. The characterization techniques developed here may be applicable to other thermogelling systems. Medicine An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment Hao Wu1, Ana R. Rodriguez2, Bernd W. Spur2, Venkat Venkataraman1,2 1Graduate School of Biomedical Sciences, Rowan University, 2Department of Cell Biology, Rowan University School of Osteopathic Medicine A low-cost, easy-to-use and powerful system is established to evaluate potential treatments that could ameliorate blood retinal barrier breach induced by histamine. Blood vessel leakage, Müller cell activation and the continuity of neuronal processes are utilized to assess the damage response and its reversal with a potential drug, lipoxin A4. Bioengineering Designing Silk-silk Protein Alloy Materials for Biomedical Applications Xiao Hu1,2,3, Solomon Duki1, Joseph Forys1, Jeffrey Hettinger1,2, Justin Buchicchio1, Tabbetha Dobbins1,2, Catherine Yang2,4 1Department of Physics and Astronomy, Rowan University, 2Department of Biomedical and Translational Sciences, Rowan University, 3Department of Biomedical Sciences, Cooper Medical School of Rowan University, 4Department of Chemistry and Biochemistry, Rowan University Blending is an efficient approach to generate biomaterials with a broad range of properties and combined features. By predicting the molecular interactions between different natural silk proteins, new silk-silk protein alloy platforms with tunable mechanical resiliency, electrical response, optical transparency, chemical processability, biodegradability, or thermal stability can be designed.