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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
JoVE Journal
Genetics
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JoVE Journal Genetics
Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
DOI:

09:45 min

August 10, 2017

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Chapters

  • 00:05Title
  • 00:53Preparation Prior to Inducing Hypoxia
  • 03:52Inducing Hypoxia and Collecting Samples
  • 07:37Results: Gene Expression Changes During the Hypoxic Response of S. cerevisiae
  • 09:12Conclusion

Summary

Automatic Translation

Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular response.

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