Oklahoma Medical Research Foundation 5 articles published in JoVE Immunology and Infection Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils Sunitha Pulikkot1, Meng Zhao2,3, Zhichao Fan1 1Department of Immunology, School of Medicine, UConn Health, 2Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, 3Department of Microbiology and Immunology, University of Oklahoma Health Science Center We describe stepwise protocols measuring the mitochondrial respiration of mouse and human neutrophils and HL60 cells using the metabolic extracellular flux analyzer. Biology Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome Sarah R. Ocañas*1,2,3, José V. V. Isola*1,3, Tatiana D. Saccon1, Kevin D. Pham2, Ana J. Chucair-Elliott2, Augusto Schneider4, Willard M. Freeman2,3, Michael B. Stout1,3 1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas In this protocol, the translating ribosome affinity purification (TRAP) method and the isolation of nuclei tagged in specific cell types (INTACT) method were optimized for the paired interrogation of the cell-specific ovarian transcriptome and epigenome using the NuTRAP mouse model crossed to a Cyp17a1-Cre mouse line. Genetics Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System Joseph C. Siefert1,2, Emily A. Clowdus1,2, Duane Goins1, Amnon Koren3, Christopher L. Sansam1,2 1Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, 2Department of Cell Biology, University of Oklahoma Health Sciences Center, 3Department of Molecular Biology and Genetics, Cornell University Zebrafish were recently used as an in vivo model system to study DNA replication timing during development. Here is detailed the protocols for using zebrafish embryos to profile replication timing. This protocol can be easily adapted to study replication timing in mutants, individual cell types, disease models, and other species. Immunology and Infection A Method for the Measurement of Salivary Gland Function in Mice Harini Bagavant1, Marta Trzeciak1, Joanna Papinska1, Indranil Biswas1, Micah L Dunkleberger1, Anna Sosnowska1, Umesh S Deshmukh1 1Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation Salivary gland hypofunction is a frequent consequence of autoimmune disease and radiation therapy. Reproducible evaluation of salivary gland function in mouse models of these diseases is a technical challenge. Here, a simple method for accurate and reproducible measurement of saliva production in mice is described. Biology Infinium Assay for Large-scale SNP Genotyping Applications Adam J. Adler1, Graham B. Wiley1, Patrick M. Gaffney1 1Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation A protocol is described that uses Illumina's Infinium assays to perform large-scale genotyping. These assays can reliably genotype millions of SNPs across hundreds of individual DNA samples in three days. Once generated, these genotypes can be used to check for associations with a variety of different diseases or phenotypes.
