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JoVE Journal Biology
Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome
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Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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Summary
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In this protocol, the translating ribosome affinity purification (TRAP) method and the isolation of nuclei tagged in specific cell types (INTACT) method were optimized for the paired interrogation of the cell-specific ovarian transcriptome and epigenome using the NuTRAP mouse model crossed to a Cyp17a1-Cre mouse line.

Tags

Biology Mouse Ovarian Epigenome Transcriptome Technique Isolate Nucleic Acids Specific Cell Populations Cell Sorting Identify Changes Aging Advantage Epigenomic Analysis Transcriptomic Analysis Throughput Cost Specialized Equipment Ovarian Tissue Cell Type-specific Analysis Reproductive Senescence Female Infertility Cre Driver Validation Ovary Collection Nuclei Lysis Buffer Micro Scissors Glass Dounce Homogenizer
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