2 articles published in JoVE
Juxtacellular Monitoring and Localization of Single Neurons within Sub-cortical Brain Structures of Alert, Head-restrained Rats Jeffrey D. Moore1, Martin Deschênes2, David Kleinfeld1 1Department of Physics 0374, University of California, San Diego, 2Department of Psychiatry and Neuroscience, Centre de Recherche de l’Université Laval Robert-Giffard This protocol describes the design and surgical implantation of a head-restraining mechanism to monitor neuronal activity in sub-cortical brain structures in alert rats. It delineates procedures to isolate single neurons in the juxtacellular configuration and to efficiently identify their anatomical locations.
Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain Jivan Khlghatyan1, Armen Saghatelyan1 1The Cellular Neurobiology Unit, Centre de Recherche Université Laval Robert-Giffard We describe a protocol for real-time videoimaging of neuronal migration in the mouse forebrain. The migration of virally-labeled or grafted neuronal precursors was recorded in acute live slices using wide-field fluorescent imaging with a relatively rapid acquisition interval to study the different phases of cell migration, including the durations of the stationary and migration phases and the speed of migration.