VA Connecticut Healthcare Systems, West Haven 4 articles published in JoVE Medicine Murine Model of Central Venous Stenosis using Aortocaval Fistula with an Outflow Stenosis Toshihiko Isaji1,2,3, Shun Ono1,2,4,5, Takuya Hashimoto1,2,3, Kota Yamamoto1,2,3, Ryosuke Taniguchi1,2,3, Haidi Hu1,2, Tun Wang1,2, Jun Koizumi4, Toshiya Nishibe5, Katsuyuki Hoshina3, Alan Dardik1,2,6 1Department of Surgery, Yale University, 2Vascular Biology and Therapeutics Program, Yale University, 3Department of Vascular Surgery, University of Tokyo, 4Department of Diagnostic Radiology, Tokai University School of Medicine, 5Department of Cardiovascular Surgery, Tokyo Medical University, 6Department of Vascular Surgery, VA Connecticut Healthcare Systems An aortocaval fistula was created by puncturing the murine infra-renal aorta through both walls into the inferior vena cava and was followed by creation of a stenosis in its outflow via partial ligation of the inferior vena cava. This reproducible model can be used to study central venous stenosis. Medicine Patch Angioplasty in the Rat Aorta or Inferior Vena Cava Hualong Bai1,2,3,4,5, Xin Li6, Takuya Hashimoto1,2,5, Haidi Hu1,2,5, Trenton R. Foster1,2,5, Jesse J. Hanisch1,2,5, Jeans M. Santana1,2,5, Alan Dardik1,2,5 1Department of Surgery, Yale University, 2Vascular Biology and Therapeutics Program, Yale University, 3Department of Vascular Surgery, First Affiliated Hospital of Zhengzhou University, 4Basic Medical College of Zhengzhou University, 5VA Connecticut Healthcare Systems, West Haven, CT, 6Department of Vascular Surgery, Xiangya Second Hospital of Central South University, Changsha, China We have established a model of pericardial patch angioplasty that can be used in either small-diameter veins or arteries. This model can be used to compare venous and arterial neointimal hyperplasia formation. Medicine Intraluminal Drug Delivery to the Mouse Arteriovenous Fistula Endothelium Takuya Hashimoto1,2,3, Kota Yamamoto1,2,3, Trenton Foster1, Hualong Bai1, Kunihiro Shigematsu4, Alan Dardik1,3 1Department of Surgery and the Vascular Biology and Therapeutics Program, Yale University, 2Department of Vascular Surgery, University of Tokyo, 3Department of Vascular Surgery, VA Connecticut Healthcare Systems, 4Department of Vascular Surgery, International University of Health and Welfare Mita Hospital After puncturing the aorta through the inferior vena cava (IVC) to create an aorto-caval fistula in the mouse, solution containing a drug is infused into the IVC via the same needle, followed by incubation. This method enables more robust drug delivery to the venous endothelium compared to the external route. Medicine Technical Aspects of the Mouse Aortocaval Fistula Kota Yamamoto1,2, Xin Li1,3, Chang Shu3, Tetsuro Miyata2, Alan Dardik1,4 1The Department of Surgery and the Interdepartmental Program in Vascular Biology and Therapeutics, Yale University, 2Department of Vascular Surgery, The University of Tokyo, 3Department of Vascular Surgery, Central South University, 4VA Connecticut Healthcare Systems The goal is to produce an arteriovenous fistula that is simple and reproducible. This method does not use sutures or glue adhesive. Therefore the samples can be used with the least amount of foreign materials for analysis.
