1 article published in JoVE

• Biology

  
  Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue Charmaine Y. Pietersen¹, Maribel P. Lim¹, Tsung-Ung W. Woo^{1,2,3 1}Department of Structural and Molecular Neuroscience, McLean Hospital, ²Department of Psychiatry, Harvard Medical School, ³Department of Psychiatry, Beth Israel Deaconess Medical Center We describe a process using laser-capture microdissection to isolate and extract RNA from a homogeneous cell population, pyramidal neurons, in layer III of the superior temporal gyrus in postmortem human brains. We subsequently linearly amplify (T7-based) mRNA, and hybridize the sample to the Affymetrix human X3P microarray.