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4 articles published in JoVE
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Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77
Pierre Leblanc1, Minho Moon1,2, Woori Kim1, Inhye Jeong1, Chun-Hyung Kim1, Kwang-Soo Kim1
1Molecular Neurobiology Laboratory, Psychiatry, McLean Hospital, 2Department of Biochemistry, College of Medicine, Konyang University
Here, we selectively target antibodies against a specific member of a highly conserved family of proteins by immunizing animals with their most divergent regions followed by removing cross reactive antibodies by pre-adsorption.
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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
Charmaine Y. Pietersen1, Maribel P. Lim1, Tsung-Ung W. Woo1,2,3
1Department of Structural and Molecular Neuroscience, McLean Hospital, 2Department of Psychiatry, Harvard Medical School, 3Department of Psychiatry, Beth Israel Deaconess Medical Center
We describe a process using laser-capture microdissection to isolate and extract RNA from a homogeneous cell population, pyramidal neurons, in layer III of the superior temporal gyrus in postmortem human brains. We subsequently linearly amplify (T7-based) mRNA, and hybridize the sample to the Affymetrix human X3P microarray.
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