3 articles published in JoVE
Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain Emily D. Geyer*1, Prithvi A. Shetty*1, Christopher J. Suozzi*1, David Z. Allen*1,2, Pamela P. Benavidez*1,2, Joseph Liu*1,3, Charles N. Hollis1, Greg A. Gerhardt4, Jorge E. Quintero4, Jason J. Burmeister4, Emmett E. Whitaker1,3 1Department of Anesthesiology, Ohio State University College of Medicine, 2Medical Student Research Program, Ohio State University College of Medicine, 3 This study explores the novel use of enzyme-based microelectrode array (MEA) technology to monitor in vivo neurotransmitter activity in piglets. The hypothesis was that glutamate dysregulation contributes to the mechanism of anesthetic neurotoxicity. Here, we present a protocol to adapt MEA technology to study the mechanism of anesthesia-induced neurotoxicity.
Use of a Piglet Model for the Study of Anesthetic-induced Developmental Neurotoxicity (AIDN): A Translational Neuroscience Approach Emmett E. Whitaker1,2, Christopher Z. Zheng1, Bruno Bissonnette1,2,3, Andrew D. Miller4, Tanner L. Koppert1,2, Joseph D. Tobias1,2, Christopher R. Pierson5,6, Fedias L. Christofi1 1Department of Anesthesiology, Ohio State University College of Medicine, 2 Anesthesia-induced developmental neurotoxicity (AIDN) research has focused on rodents, which are not broadly applicable to humans. Non-human primate models are more relevant, but are cost-prohibitive and difficult to use for experimentation. The piglet, in contrast, is a clinically relevant, practical animal model ideal for the study of anesthetic neurotoxicity.
In vitro Biofilm Formation in an 8-well Chamber Slide Joseph A. Jurcisek1, Amanda C. Dickson1, Molly E. Bruggeman1, Lauren O. Bakaletz1 1Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital This article describes the procedure for the formation and visualization of a bacterial biofilm grown within an 8-well chamber slide