3 articles published in JoVE
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea Rabab Sharif1, Shrestha Priyadarsini2, Tyler G. Rowsey2, Jian-Xing Ma3, Dimitrios Karamichos1,2 1Department of Cell Biology, University of Oklahoma Health Sciences Center, 2Department of Ophthalmology/Dean McGee Eye Institute, University of Oklahoma Health Sciences Center, 3Department of Physiology, Harold Hamm Diabetes Center, University of Oklahoma Health Sciences Center This protocol describes a novel three-dimensional in vitro model, where corneal stromal cells and differentiated neuronal cells are cultured together to assist in the examination and understanding of the interactions of the two cell types.
An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants Yuhui Chen1, Xianfu Wang2, Shunfei Lu3, Hongcheng Wang2, Shibo Li2, Rujin Chen1 1Laboratory of Plant Genetics and Development, Noble Research Institute, 2Genetics Laboratory, University of Oklahoma Health Science Center, 3Medicine and Health School, Li Shui University This protocol provides experimental steps and information about reagents, equipment, and analysis tools for researchers who are interested in carrying out whole genome array-based comparative genomic hybridization (CGH) analysis of copy number variations in plants.
Disposable Dosators for Pulmonary Insufflation of Therapeutic Agents to Small Animals Phillip G. Durham1, Shumaila N. Hanif2, Lucia Garcia Contreras2, Ellen F. Young3, Miriam S. Braunstein3, Anthony J. Hickey1 1RTI International, 2Oklahoma University Health Science Center, 3University of North Carolina at Chapel Hill During development of drugs for pulmonary delivery, it is necessary to evaluate pharmacokinetics and efficacy in an animal model. We present a method to build a disposable aerosol dispersion system from of-the-shelf components that can be used to administer intrapulmonary dry powder aerosol to rodents.