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Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
DOI:

07:24 min

February 06, 2021

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Chapters

  • 00:04Introduction
  • 00:54Bacillus cereus Culture and Dilution
  • 01:48Mouse Intravitreal Injection
  • 04:02Eye Harvest
  • 04:43Intraocular Bacterial Count
  • 06:06Results: Representative Bacterial Endophthalmitis Mouse Model Validation
  • 06:47Conclusion

Summary

Automatic Translation

We describe here a method of intravitreal injection and subsequent bacterial quantitation in mouse model of bacterial endophthalmitis. This protocol can be extended for measuring host immune responses and bacterial and host gene expression.

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