Children's Hospital of Pittsburgh of UPMC
4 articles published in JoVE
Pancreatic Duct Infusion: An Effective and Selective Method of Drug and Viral Delivery Joseph C. Fusco*1, Chen Congde*2, Xiangwei Xiao1, Krishna Prasadan1, David Ricks1, George K. Gittes1 1 Pancreatic duct infusion is an important technique that can allow for lineage tracing, gene introduction, and cell line-specific targeting. A pancreatic duct infusion technique for drug and viral delivery to pancreatic cells is presented here.
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing Ayelet Di Segni1, Tzipi Braun1, Marina BenShoshan1, Sarit Farage Barhom1, Efrat Glick Saar1, Karen Cesarkas1, James E. Squires2, Nathan Keller1,3, Yael Haberman1,4 1Sheba Medical Center, 2 This manuscript describes a detailed standardized protocol of high-throughput 16S rRNA-amplicon sequencing. The protocol introduces an integrated, uniformed, feasible, and inexpensive protocol starting from fecal sample collection through data analyses. This protocol enables analysis of large numbers of samples with rigorous standards and several controls.
Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates Alexander G. White*1, Pauline Maiello*1, M. Teresa Coleman1, Jaime A. Tomko1, L. James Frye1, Charles A. Scanga1, Philana Ling Lin2, JoAnne L. Flynn1 1Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, 2 Here, we present a protocol to describe the analysis of 18F-FDG PET/CT imaging in non-human primates that have been infected with M. tuberculosis to study disease process, drug treatment, and disease reactivation.
In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow Christopher C. Rymer1,2, Sunder Sims-Lucas1,2 1 This manuscript describes a technique for visualization of the developing vasculature. Here we utilized in utero intra-cardiac FITC-labeled tomato lectin microinjections on mouse embryos. Using this technique, we delineate the perfused and unperfused vessels throughout the embryonic kidney.