Children's Hospital of Pittsburgh of UPMC

4 articles published in JoVE

• Medicine

  
  Pancreatic Duct Infusion: An Effective and Selective Method of Drug and Viral Delivery Joseph C. Fusco*¹, Chen Congde*², Xiangwei Xiao¹, Krishna Prasadan¹, David Ricks¹, George K. Gittes^{1 1} Pancreatic duct infusion is an important technique that can allow for lineage tracing, gene introduction, and cell line-specific targeting. A pancreatic duct infusion technique for drug and viral delivery to pancreatic cells is presented here.

• Biology

  
  Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing Ayelet Di Segni¹, Tzipi Braun¹, Marina BenShoshan¹, Sarit Farage Barhom¹, Efrat Glick Saar¹, Karen Cesarkas¹, James E. Squires², Nathan Keller^1,3, Yael Haberman^{1,4 1}Sheba Medical Center, ² This manuscript describes a detailed standardized protocol of high-throughput 16S rRNA-amplicon sequencing. The protocol introduces an integrated, uniformed, feasible, and inexpensive protocol starting from fecal sample collection through data analyses. This protocol enables analysis of large numbers of samples with rigorous standards and several controls.

• Immunology and Infection

  
  Analysis of ¹⁸FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates Alexander G. White*¹, Pauline Maiello*¹, M. Teresa Coleman¹, Jaime A. Tomko¹, L. James Frye¹, Charles A. Scanga¹, Philana Ling Lin², JoAnne L. Flynn^{1 1}Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, ² Here, we present a protocol to describe the analysis of ¹⁸F-FDG PET/CT imaging in non-human primates that have been infected with M. tuberculosis to study disease process, drug treatment, and disease reactivation.

• Developmental Biology

  
  In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow Christopher C. Rymer^1,2, Sunder Sims-Lucas^{1,2 1} This manuscript describes a technique for visualization of the developing vasculature. Here we utilized in utero intra-cardiac FITC-labeled tomato lectin microinjections on mouse embryos. Using this technique, we delineate the perfused and unperfused vessels throughout the embryonic kidney.