Journal
/
Medicine
/
Pancreatic Duct Infusion: An Effective and Selective Method of Drug and Viral Delivery
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Pancreatic Duct Infusion: An Effective and Selective Method of Drug and Viral Delivery

Pancreatic Duct Infusion: An Effective and Selective Method of Drug and Viral Delivery

DOI:
10.3791/55332-v

•

06:03 min

•

September 30, 2021

•

, , , , ,
1Department of Pediatric Surgery,Children’s Hospital of Pittsburgh of UPMC, 2Department of Pediatric Surgery,The 2nd Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University

Chapters

  • 00:04Title
  • 00:31Preoperative Preparation
  • 01:15Proper Exposure and Duodenotomy
  • 02:18Infusion
  • 03:47Sample Preparation
  • 04:37Results: Pancreas-specific Expression of GFP Following Pancreatic Duct Infusion of AAV8
  • 05:14Conclusion

Summary

Automatic Translation

Automatic Translation

Pancreatic duct infusion is an important technique that can allow for lineage tracing, gene introduction, and cell line-specific targeting. A pancreatic duct infusion technique for drug and viral delivery to pancreatic cells is presented here.

Tags

Pancreatic Duct InfusionLineage TracingGene IntroductionCell Line-specific TargetingChronic PancreatitisDiabetesPancreatic CancerAnesthetized MouseDuodenumPancreatic DuctInfusion PumpCatheterBulldog Vascular ClampSaline Injection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Surgical Injury to the Mouse Pancreas through Ligation of the Pancreatic Duct as a Model for Endocrine and Exocrine Reprogramming and Proliferation

An Improved Method for Rapid Intubation of the Trachea in Mice

Intraluminal Drug Delivery to the Mouse Arteriovenous Fistula Endothelium

Drug-Induced Sleep Endoscopy (DISE) with Target Controlled Infusion (TCI) and Bispectral Analysis in Obstructive Sleep Apnea

A Novel Method: Super-selective Adrenal Venous Sampling

Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

Robotic Enucleation of an Intra-Pancreatic Insulinoma in the Pancreatic Head

External Cephalic Version: Is it an Effective and Safe Procedure?

Read Article