Scientific Platform Pasteur USP 1 article published in JoVE Immunology and Infection High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions André Nicolau Aquime Gonçalves1,2, Vanessa Escolano Maso3, Ícaro Maia Santos de Castro2,3, Amanda Pereira Vasconcelos3, Rodrigo Luiz Tomio Ogava2,3, Helder I Nakaya2,3,4 1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School, University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, 4Hospital Israelita Albert Einstein The protocol presented here describes a complete pipeline to analyze RNA-sequencing transcriptome data from raw reads to functional analysis, including quality control and preprocessing steps to advanced statistical analytical approaches.
